Bristol Institute for Transfusion Sciences, NHS Blood and Transplant, UK.
Vox Sang. 2011 Nov;101(4):327-32. doi: 10.1111/j.1423-0410.2011.01540.x. Epub 2011 Sep 6.
The fourth International Society of Blood Transfusion (ISBT) workshop on molecular blood group genotyping was held in 2010, with a feedback meeting at the ISBT Congress in Berlin, Germany. Fifty laboratories participated, 17 more than in 2008. Six samples were distributed. Samples 1-3 were DNA samples for all red cell blood group tests available to the participants. Of the 46 laboratories that tested these samples, 37 obtained completely correct results, although the extent of testing varied considerably. Sample 4, also a DNA sample, was an Rh problem in which RHDΨ and RHCE*ceCF were present, but the participants were only informed that the donor's red cells typed as positive with some monoclonal anti-D. Of the 42 laboratories that participated in this exercise, seven performed the sequencing necessary to obtain the correct result. Samples 5 and 6 were plasma samples from RhD-negative pregnant women, for foetal RhD testing. These were sent to 25 laboratories, and two incorrect results were reported. Overall, the level of accuracy was about equal to that of the previous workshop. The main conclusion for the last two workshops can be reiterated: with greater care and attention to detail, very high standards could be set for molecular blood group genotyping.
第四届国际输血协会（ISBT）分子血型基因分型研讨会于 2010 年在德国柏林召开，会上还举行了 ISBT 大会的反馈会议。共有 50 个实验室参加，比 2008 年增加了 17 个。会上分发了 6 个样本。样本 1-3 是供所有与会者进行所有红细胞血型检测的 DNA 样本。在检测这些样本的 46 个实验室中，有 37 个实验室得到了完全正确的结果，尽管检测的范围差异很大。样本 4 也是一个 DNA 样本，是一个 Rh 问题，其中存在 RHDΨ 和 RHCE*ceCF，但参与者只被告知供体的红细胞与一些单克隆抗-D 呈阳性反应。在参与这项研究的 42 个实验室中，有 7 个实验室进行了测序以获得正确的结果。样本 5 和 6 是 RhD 阴性孕妇的血浆样本，用于胎儿 RhD 检测。这两个样本被送到 25 个实验室，报告了两个错误的结果。总的来说，准确性水平与前一届研讨会相当。最后两届研讨会的主要结论可以重申：如果更加小心和注重细节，可以为分子血型基因分型设定非常高的标准。
