INSERM U, Department of Urology, Lille University Hospital, University Lille Nord de France, France.
BJU Int. 2012 Feb;109(3):452-8. doi: 10.1111/j.1464-410X.2011.10406.x. Epub 2011 Sep 2.
To examine the feasibility and reproducibility of laser interstitial thermotherapy (LITT) as a minimally invasive method for the treatment of prostate cancer.
Heterotopic tumours of prostatic adenocarcinoma (Dunning R3327-AT2) were induced in 10 male Copenhagen rats. After preoperative magnetic resonance imaging (MRI), a 10-mm cylindrical diffusing fibre developed by our research department was inserted under ultrasonographic guidance into the tumour. LITT was performed with a 980-nm diode laser (power 5 W) for 75 s (fluence rate of 1145 J/cm(2)). Non-enhanced T2-weighted and dynamic gadolinium-enhanced T1-weighted MRI examinations were performed at baseline, 1 and 48 h after the procedure and correlated with histological findings.
The necrosis lesions induced by LITT were visible on MRI. The mean (SD) ellipsoid necrosis volumes were 0.748 (0.075) mL at 1 h and 0.982 (0.052) mL at 48 h after the LITT procedure, and significantly different (P < 0.001). Histological analysis showed a strong correlation (r = 0.87) with the mean necrosis volume obtained by MRI at 48 h after LITT.
In a prostatic adenocarcinoma model, 980-nm LITT induces reproducible necrosis volumes. Further characterization of the response to LITT in an animal model and in human tissues will be important in establishing the efficacy of the procedure for prostate cancer focal therapy.
研究激光间质热疗（LITT）作为一种治疗前列腺癌的微创方法的可行性和可重复性。
在 10 只雄性 Copenhagen 大鼠中诱导前列腺腺癌（Dunning R3327-AT2）异位肿瘤。在术前磁共振成像（MRI）后，在超声引导下将我们研究部门开发的 10mm 圆柱形弥散纤维插入肿瘤中。使用 980nm 二极管激光（功率 5W）进行 LITT 治疗 75s（能量密度为 1145J/cm2）。在手术前、手术后 1 小时和 48 小时进行非增强 T2 加权和动态钆增强 T1 加权 MRI 检查，并与组织学发现相关联。
LITT 诱导的坏死病变在 MRI 上可见。LITT 手术后 1 小时和 48 小时的平均（标准差）椭圆坏死体积分别为 0.748（0.075）mL 和 0.982（0.052）mL，差异有统计学意义（P<0.001）。组织学分析显示与 LITT 后 48 小时 MRI 获得的平均坏死体积具有很强的相关性（r=0.87）。
在前列腺腺癌模型中，980nm LITT 可诱导可重复的坏死体积。在动物模型和人类组织中进一步描述 LITT 反应的特征对于确定该程序治疗前列腺癌的疗效非常重要。
