Department of Experimental Medicine, Sapienza University, Rome, Italy.
Int J Lab Hematol. 2012 Apr;34(2):154-63. doi: 10.1111/j.1751-553X.2011.01373.x. Epub 2011 Sep 6.
Accurate white blood cell counting (WBC) and differential count by blood analyzers could allow a more informative characterization of granulocyte colony-stimulating factor (G-CSF) mobilized blood (MB), leukapheresis products (LP), and cord blood (CB). However, reliable counting by a blood cell analyzer in this setting is a major challenge owing to quali-quantitative abnormalities of blood cells.
We evaluated the performances of the analyzer Pentra DX 120 by Horiba ABX working with dedicated cell-gating profiles, which generate three-part differential counts in samples obtained from donors' MB, LP, and CB. The results of the analyzer were compared to counts obtained by flow cytometry and manual counts, the latter performed for reference validation and in the case of discrepant results between study and reference counts.
Pentra DX 120 generated highly correlated counts (R > 0.91 in all cases) to those obtained by flow cytometry in all samples (MB, LP, and CB) with high degree of count accuracy in most cases and referred to WBC absolute count and differential count including lymphocytes (LYM) %, monocytes (MON) %, and polymorphonuclear leukocytes (PMN) %. Accuracy, judged by the difference between study and reference counts and expressed as percentage of reference count, ranged from 0.8% to 8.6%, and sporadic loss of accuracy occurred for MON % only in no more than 10% of CB samples.
The ABX Pentra DX 120 provided accurate WBC count and differential count during MB, LP, and CB analyses and allowed a better characterization of donors' hematologic status and graft composition.
通过血液分析仪进行准确的白细胞计数（WBC）和分类计数，可以更详细地描述粒细胞集落刺激因子（G-CSF）动员的血液（MB）、白细胞分离产物（LP）和脐带血（CB）。然而，由于血细胞的质和量的异常，在这种情况下，血液细胞分析仪的可靠计数是一个主要的挑战。
我们评估了 Horiba ABX 公司的 Pentra DX 120 分析仪的性能，该分析仪与专用的细胞门控分析程序一起使用，可在从供体 MB、LP 和 CB 获得的样本中生成三分类差异计数。分析仪的结果与流式细胞术和手动计数的结果进行了比较，后者用于参考验证，并且在研究计数与参考计数不一致的情况下进行。
在所有样本（MB、LP 和 CB）中，Pentra DX 120 与流式细胞术生成的高度相关计数（所有情况下 R > 0.91），在大多数情况下具有高度的计数准确性，并参考 WBC 绝对值计数和分类计数，包括淋巴细胞（LYM）%、单核细胞（MON）%和多形核白细胞（PMN）%。准确性通过研究计数与参考计数之间的差异来判断，并表示为参考计数的百分比，范围从 0.8%到 8.6%，只有 MON%在不超过 10%的 CB 样本中偶尔会出现准确性下降。
ABX Pentra DX 120 在 MB、LP 和 CB 分析中提供了准确的 WBC 计数和分类计数，并允许更好地描述供体的血液学状态和移植物成分。
