Department of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Korea.
Int J Lab Hematol. 2013 Dec;35(6):658-65. doi: 10.1111/ijlh.12110. Epub 2013 Jun 6.
Cord blood (CB) is an important source of hematopoietic stem cells and reflects the hematologic status of neonates. ABX Pentra DX 120 (Horiba Medical, Montpellier, France) and Sysmex XE-2100 (Sysmex, Kobe, Japan) were compared in 200 CB specimens.
Complete blood count parameters including white blood cell (WBC) differential counts were compared between the two analyzers. Double differential matrix (DDX) by ABX Pentra DX 120 and hematopoietic progenitor cell (HPC) by Sysmex XE-2100 were compared with CD34(+) cells by flow cytometry.
Most of the parameters showed acceptable correlation between the two analyzers. Although WBC differential of both analyzers showed acceptable correlation with manual counts, mononuclear cells (MNC) by ABX Pentra DX 120 better correlated with manual count than MNC by Sysmex XE-2100. NRBC by Sysmex XE-2100 better correlated with manual count than NRBC by ABX Pentra DX 120. ABX Pentra DX 120 showed better flagging performances. DDX better correlated with CD34(+) cells than HPC.
Although the results from both analyzers are mostly interchangeable and reliable in CB specimens, flagging performance of ABX Pentra DX 120 seems to be superior to that of Sysmex XE-2100. DDX by ABX Pentra DX 120 would be valuable to evaluate the quality of CB for further therapeutic utilization.
脐带血（CB）是造血干细胞的重要来源，反映了新生儿的血液学状态。ABX Pentra DX 120（法国蒙彼利埃的Horiba Medical）和 Sysmex XE-2100（日本神户的Sysmex）在 200 例 CB 标本中进行了比较。
比较了两种分析仪之间的全血细胞计数参数，包括白细胞（WBC）差异计数。ABX Pentra DX 120 的双差矩阵（DDX）和 Sysmex XE-2100 的造血祖细胞（HPC）与流式细胞术的 CD34(+)细胞进行了比较。
大多数参数在两种分析仪之间显示出可接受的相关性。虽然两种分析仪的 WBC 差异均与手工计数显示出可接受的相关性，但 ABX Pentra DX 120 的单核细胞（MNC）比 Sysmex XE-2100 的 MNC 与手工计数的相关性更好。NRBC 由 Sysmex XE-2100 比 ABX Pentra DX 120 与手动计数的相关性更好。ABX Pentra DX 120 显示出更好的标记性能。DDX 与 CD34(+)细胞的相关性优于 HPC。
尽管两种分析仪的结果在 CB 标本中大多是可互换且可靠的，但 ABX Pentra DX 120 的标记性能似乎优于 Sysmex XE-2100。ABX Pentra DX 120 的 DDX 有助于评估 CB 的质量，以进一步用于治疗。
