International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.
Plant Signal Behav. 2011 Oct;6(10):1447-50. doi: 10.4161/psb.6.10.17106. Epub 2011 Oct 1.
Helicases are motor proteins that can transiently catalyze the unwinding of energetically stable duplex DNA or RNA molecules by using ATP hydrolysis as the source of energy. Many helicases share a core region of highly conserved sequence motifs, and belong to the rapidly growing DEAD-box protein family. Pea DNA helicase 45 (PDH45), that exhibits striking homology with eukaryotic translation initiation factor 4A (eIF4A), contains ATP-dependent DNA and RNA helicase, DNA-dependent ATPase, and ATP-binding activities. The transcript of the PDH45 gene was reported to be upregulated in pea plant in response to high salinity, cold stress, abscisic acid (ABA), dehydration and early wounding. The first direct evidence that overexpression of PDH45 confers salinity stress tolerance without yield loss has also been reported. A promoter analysis of PDH45 gene has not been studied. The cis-regulatory elements present on promoter region of the gene act as binding sites for RNA polymerase and transcription factors and control the regulation of gene expression. Here we report the promoter of the PDH45 gene that contains stress-responsive cis-regulatory elements which may be responsible for regulating the expression of PDH45 under abiotic stress conditions.
解旋酶是一种能够利用 ATP 水解作为能量来源，暂时催化能量稳定的双链 DNA 或 RNA 分子解旋的运动蛋白。许多解旋酶共享高度保守序列基序的核心区域，并且属于快速增长的 DEAD 框蛋白家族。豌豆 DNA 解旋酶 45（PDH45）与真核翻译起始因子 4A（eIF4A）表现出显著的同源性，它包含 ATP 依赖性 DNA 和 RNA 解旋酶、DNA 依赖性 ATP 酶和 ATP 结合活性。据报道，PDH45 基因的转录物在豌豆植物中对高盐度、冷胁迫、脱落酸（ABA）、干旱和早期创伤做出反应而上调。也有报道称，过表达 PDH45 可以在不降低产量的情况下赋予耐盐性，这是第一个直接证据。尚未研究 PDH45 基因的启动子分析。基因启动子区域存在的顺式调控元件作为 RNA 聚合酶和转录因子的结合位点，控制基因表达的调节。在这里，我们报告了 PDH45 基因的启动子，其中包含应激反应性顺式调控元件，这些元件可能负责调节非生物胁迫条件下 PDH45 的表达。
