Gupta Ashish, Chandra Tulika, Kumar Ashutosh
Department of Transfusion Medicine and Blood Bank, Chhatrapati Shahuji Maharaj Medical University, Lucknow, Uttar Pradesh, India.
Asian J Transfus Sci. 2011 Jul;5(2):160-3. doi: 10.4103/0973-6247.83244.
Platelets are routinely isolated from whole blood and stored in plasma for 5 days. This study was done to assess the in vitro function of random donor platelets stored for 7 days in composol platelet additive solution at 22°C.
The study sample included 30 blood donors of both sex in State Blood Bank, C S M Medical University, Lucknow. Random donor platelets were prepared by the platelet-rich plasma method. Whole blood (350 ml) was collected in anticoagulant Citrate Phosphate Dextrose Adenine triple blood bags. Random donor platelets were stored for 7 days at 22°C in platelet incubators and agitators with and without additive solution.
Platelet swirling was present in all the units at 22°C on day 7 with no evidence of bacterial contamination. Comparison of the mean values of platelet count, platelet factor 3, lactate dehydrogenase, pH, glucose and platelet aggregation showed no significant difference in additive solution while platelet factor 3, glucose and platelet aggregation showed significant difference (P < 0.001) on day 7 without additive solution at 22°C.
Our study infers that the platelet viability and aggregation were the best maintained within normal levels on day 7 of storage in platelet additive solution at 22°C. Thus, we may conclude that in vitro storage of random donor platelets with an extended shelf life of 7 days using platelet additive solution may be advocated to improve the inventory of platelets.
血小板通常从全血中分离出来，并在血浆中储存5天。本研究旨在评估随机供体血小板在22°C下于复方血小板添加剂溶液中储存7天的体外功能。
研究样本包括勒克瑙市CSM医学院国家血库的30名男女献血者。通过富血小板血浆法制备随机供体血小板。将350ml全血采集到抗凝剂枸橼酸盐磷酸盐葡萄糖腺嘌呤三联血袋中。随机供体血小板在22°C的血小板培养箱和搅拌器中，分别在有和没有添加剂溶液的情况下储存7天。
在第7天，所有样本中的血小板在22°C时均出现漩涡现象，且无细菌污染迹象。血小板计数、血小板因子3、乳酸脱氢酶、pH值、葡萄糖和血小板聚集的平均值比较显示，在添加剂溶液中无显著差异，而在22°C无添加剂溶液的情况下，第7天时血小板因子3、葡萄糖和血小板聚集有显著差异（P<0.001）。
我们的研究推断，在22°C下于血小板添加剂溶液中储存7天时，血小板活力和聚集在正常水平内保持最佳。因此，我们可以得出结论，使用血小板添加剂溶液将随机供体血小板的体外储存保质期延长至7天，可能有助于改善血小板库存。
