Thiruppathiraja Chinnasamy, Saroja Veerappan, Kamatchiammal Senthilkumar, Adaikkappan Periyakaruppan, Alagar Muthukaruppan
Nanocomposites Research group, Department of Chemical Engineering, Anna University, Chennai, TN, India.
J Environ Monit. 2011 Oct;13(10):2782-7. doi: 10.1039/c1em10372e. Epub 2011 Sep 7.
Cryptosporidium parvum is one of the most important biological contaminants in drinking water and generates significant risks to public health. Due to low infectious dose of C. parvum, remarkably sensitive detection methods are required for water and food industry analysis. This present study describes a simple, sensitive, enzyme amplified sandwich form of an electrochemical immunosensor using dual labeled gold nanoparticles (alkaline phosphatase and anti-oocysts monoclonal antibody) in indium tin oxide (ITO) as an electrode to detect C. parvum. The biosensor was fabricated by immobilizing the anti-oocysts McAb on a gold nanoparticle functionalized ITO electrode, followed by the corresponding capture of analytes and dual labeled gold nanoparticle probe to detect the C. parvum target. The outcome shows the sensitivity of electrochemical immune sensor enhanced by gold nanoparticles with a limit of detection of 3 oocysts/mL in a minimal processing period. Our results demonstrated the sensitivity of the new approach compared to the customary method and the immunosensors showed acceptable precision, reproducibility, stability, and could be readily applied to multi analyte determination for environmental monitoring.
微小隐孢子虫是饮用水中最重要的生物污染物之一，对公众健康构成重大风险。由于微小隐孢子虫的感染剂量低，水和食品行业分析需要极其灵敏的检测方法。本研究描述了一种简单、灵敏的酶放大夹心型电化学免疫传感器，该传感器使用双标记金纳米颗粒（碱性磷酸酶和抗卵囊单克隆抗体）修饰氧化铟锡（ITO）作为电极来检测微小隐孢子虫。通过将抗卵囊单克隆抗体固定在金纳米颗粒功能化的ITO电极上制备生物传感器，随后相应地捕获分析物并使用双标记金纳米颗粒探针来检测微小隐孢子虫靶标。结果表明，金纳米颗粒增强了电化学免疫传感器的灵敏度，在最短处理时间内检测限为3个卵囊/毫升。我们的结果证明了这种新方法与传统方法相比的灵敏度，并且该免疫传感器显示出可接受的精密度、重现性、稳定性，并且可以很容易地应用于环境监测的多分析物测定。
