应用 ELISA 型丝网印刷电极伏安法检测微小隐孢子虫卵囊。
Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts.
机构信息
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia; Environmental Biotechnology CRC, Australian Technology Park, Eveleigh, NSW 2006, Australia.
出版信息
J Microbiol Methods. 2013 Nov;95(2):182-5. doi: 10.1016/j.mimet.2013.08.014. Epub 2013 Aug 29.
We report a novel electrochemical method for the rapid detection of the parasitic protozoan, Cryptosporidium parvum. An antibody-based capture format was transferred onto screen-printed electrodes and the presence of horseradish peroxidase-labelled antibodies binding to the oocysts was potentiometrically detected. This method allowed the detection of 5 × 10(2)Cryptosporidium oocysts per mL in 60 min.
我们报告了一种新型电化学方法，用于快速检测寄生原生动物微小隐孢子虫。基于抗体的捕获形式被转移到印刷电极上，并通过测定与卵囊结合的辣根过氧化物酶标记抗体的存在来进行检测。该方法允许在 60 分钟内检测到每毫升 5×10(2)个微小隐孢子虫卵囊。
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