Waap Silke, Amaral Ana R, Gomes Bruno, Manuela Coelho M
Centro de Biologia Ambiental, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisbon, Portugal.
Genetica. 2011 Aug;139(8):1009-18. doi: 10.1007/s10709-011-9602-0. Epub 2011 Sep 7.
The phylogenetic relationships of the genus Squalius are believed to be well established based on the mitochondrial cytochrome b gene. Here, we inferred the phylogenetic relationships of all species inhabiting most of the western Iberia river systems using a nuclear multi-locus approach and different species tree methods: concatenation and coalescent-based methods (BEST and minimize-deep-coalescence). The dataset comprised sequences of seven coding and three non-coding regions belonging to seven nuclear genes, which were chosen to cover multiple biological functions: amh, bmp4, ef1a, egr2, irbp, rh and rpl8. We provide evidence for a conflicting topology between the nuDNA species tree and the widely reported mtDNA gene tree. S. pyrenaicus is rendered paraphyletic in all nuDNA species trees, with populations of the Tagus/Colares clustering with S. carolitertii, while populations from the Guadiana, Sado and Almargem form a separate clade. Although a larger sampling size encompassing the full spectrum of Squalius populations in western Iberia is still needed to fully elucidate the phylogeography and species delimitation of this genus, our results suggest that the two S. pyrenaicus clades may represent different species.
基于线粒体细胞色素b基因，人们认为拟鲤属的系统发育关系已得到很好的确立。在此，我们使用核多位点方法和不同的物种树方法（串联法和基于溯祖理论的方法：BEST和最小化深度溯祖）推断了栖息于伊比利亚半岛西部大部分河流系统的所有物种的系统发育关系。数据集包含属于七个核基因的七个编码区和三个非编码区的序列，这些基因被选择用于涵盖多种生物学功能：抗缪勒氏管激素（amh）、骨形态发生蛋白4（bmp4）、延伸因子1α（ef1a）、早期生长反应蛋白2（egr2）、视网膜结合蛋白（irbp）、视黄醇结合蛋白（rh）和核糖体蛋白L8（rpl8）。我们提供了证据表明核DNA物种树与广泛报道的线粒体DNA基因树之间存在拓扑冲突。在所有核DNA物种树中，比利牛斯拟鲤（S. pyrenaicus）呈现出并系群状态，塔霍河/科拉雷斯河的种群与卡罗利特拟鲤（S. carolitertii）聚类在一起，而瓜迪亚纳河、萨多河和阿尔马任河的种群则形成一个单独的分支。尽管仍需要更大规模的采样来全面涵盖伊比利亚半岛西部拟鲤种群的全谱，以充分阐明该属的系统地理学和物种界定，但我们的结果表明，比利牛斯拟鲤的两个分支可能代表不同的物种。
