活性氧物质对离体声带上皮的急性应激。
Acute stress to excised vocal fold epithelium from reactive oxygen species.
机构信息
Communication Sciences and Disorders, University of Iowa Iowa City, Iowa, USA.
出版信息
Laryngoscope. 2011 Oct;121(10):2180-4. doi: 10.1002/lary.22157. Epub 2011 Sep 6.
OBJECTIVES/HYPOTHESIS: Vocal fold epithelium is exposed to reactive oxygen species from the inhaled environment and from tissue inflammation. The objective of this study was to explore the functional and structural consequences of reactive oxygen species exposure on vocal fold epithelium.
STUDY DESIGN
In vitro, prospective study design.
METHODS
Hydrogen peroxide (H(2)O(2)), a common reactive oxygen species, was utilized in this study. Freshly excised, viable porcine vocal fold epithelia (N = 32) were exposed to H(2) O(2) or sham challenge for 2 hours. Electrophysiology, western blotting, and light microscopy were used to quantify the functional and structural effects of reactive oxygen species on vocal fold epithelia.
RESULTS
Exposure to reactive oxygen species did not significantly alter transepithelial resistance. There was a small, nonsignificant trend for decreased concentration of epithelial junctional complex protein with reactive oxygen species challenge. Minimal changes to the gross structural appearance of vocal fold epithelia were also noted.
CONCLUSIONS
The stratified squamous epithelia of the vocal folds effectively defend against an acute reactive oxygen species challenge. The current study lays the groundwork for future investigations on the effects of reactive oxygen species on vocal fold epithelia that are compromised from phonotrauma.
目的/假设：声带上皮组织会受到吸入环境和组织炎症产生的活性氧的影响。本研究的目的是探索活性氧暴露对声带上皮组织的功能和结构的影响。
研究设计
体外前瞻性研究设计。
方法
本研究使用了过氧化氢（H₂O₂）这种常见的活性氧。将新鲜取出的、存活的猪声带上皮组织（N=32）暴露于 H₂O₂或假处理中 2 小时。用电生理学、蛋白质印迹和光学显微镜来量化活性氧对声带上皮组织的功能和结构的影响。
结果
活性氧暴露并未显著改变跨上皮电阻。活性氧处理后，上皮连接复合体蛋白的浓度略有下降，但无统计学意义。声带上皮组织的大体结构外观也仅有微小变化。
结论
声带的复层鳞状上皮组织能有效地抵御急性活性氧的挑战。本研究为未来研究活性氧对因声创伤而受损的声带上皮组织的影响奠定了基础。
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