Levendoski Elizabeth Erickson, Sivasankar M Preeti
Department of Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, IN, USA,
J Membr Biol. 2014 May;247(5):441-50. doi: 10.1007/s00232-014-9651-2. Epub 2014 Mar 20.
The vocal fold epithelium is exposed to inhaled particulates including pollutants during breathing in everyday environments. Yet, our understanding of the effects of pollutants on vocal fold epithelial function is extremely limited. The objective of this study was to investigate the effect of the pollutant acrolein on two vocal fold epithelial mechanisms: ion transport and mucin (MUC) synthesis. These mechanisms were chosen as each plays a critical role in vocal defense and in maintaining surface hydration which is necessary for optimal voice production. Healthy, native porcine vocal folds (N = 85) were excised and exposed to an acrolein or sham challenge. A 60-min acrolein, but not sham challenge significantly reduced ion transport and inhibited cyclic adenosine monophosphate-dependent, increases in ion transport. Decreases in ion transport were associated with reduced sodium absorption. Within the same timeline, no significant acrolein-induced changes in MUC gene or protein expression were observed. These results improve our understanding of the effects of acrolein on key vocal fold epithelial functions and inform the development of future investigations that seek to elucidate the impact of a wide range of pollutant exposures on vocal fold health.
在日常环境中呼吸时，声带上皮会接触到包括污染物在内的吸入颗粒物。然而，我们对污染物对声带上皮功能影响的了解极为有限。本研究的目的是调查污染物丙烯醛对两种声带上皮机制的影响：离子转运和粘蛋白（MUC）合成。选择这些机制是因为它们在声带防御以及维持表面水合作用中都起着关键作用，而表面水合作用是实现最佳发声所必需的。切除健康的天然猪声带（N = 85），并使其接受丙烯醛或假刺激。60分钟的丙烯醛刺激而非假刺激显著降低了离子转运，并抑制了环磷酸腺苷依赖性离子转运的增加。离子转运的降低与钠吸收减少有关。在同一时间范围内，未观察到丙烯醛诱导的MUC基因或蛋白质表达的显著变化。这些结果增进了我们对丙烯醛对关键声带上皮功能影响的理解，并为未来旨在阐明广泛污染物暴露对声带健康影响的研究提供了参考。
