Bolin C A, Cassells J A
Leptospirosis and Mycobacteriosis Research Unit, USDA, National Animal Disease Center, Ames, IA 50010.
J Am Vet Med Assoc. 1990 May 15;196(10):1601-4.
Leptospira interrogans serovar bratislava was isolated from a herd of swine in Iowa with a history of stillborn and weak neonatal pigs. Placentas, kidneys, and lungs of stillborn and weak pigs from 3 litters were processed to detect leptospires by use of bacteriologic culture and fluorescent antibody testing. Sera from stillborn and weak pigs were tested to detect agglutinating antibody against leptospires. A low antibody titer against L interrogans serovar bratislava was detected in the sera of stillborn and weak pigs. Small numbers of leptospires were sometimes detected in tissues by use of the fluorescent antibody test. Serovar bratislava was isolated from placentas, stillborn pigs or weak pigs from each of the 3 litters.
从爱荷华州一群有新生仔猪死产和体弱病史的猪群中分离出问号钩端螺旋体布拉迪斯拉发血清型。对来自3窝死产和体弱仔猪的胎盘、肾脏和肺进行处理,通过细菌培养和荧光抗体检测来检测钩端螺旋体。检测死产和体弱仔猪的血清以检测抗钩端螺旋体的凝集抗体。在死产和体弱仔猪的血清中检测到针对问号钩端螺旋体布拉迪斯拉发血清型的低抗体滴度。有时通过荧光抗体检测在组织中检测到少量钩端螺旋体。从3窝中的每一窝的胎盘、死产仔猪或体弱仔猪中分离出布拉迪斯拉发血清型。