State Key Laboratory for Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, China.
Mass Spectrom Rev. 2012 Jul-Aug;31(4):437-47. doi: 10.1002/mas.20346. Epub 2011 Sep 2.
Proteins play important roles in living systems and are topics of many fundamental and applied research projects. With the introduction of electrospray ionization and matrix-assisted laser desorption/ionization for analysis of biomacromolecules in the late 1980s, mass spectrometry has become an important tool for characterization of proteins. Characterization of proteins in raw samples by these mass spectrometric techniques, however, usually requires extensive sample pretreatment. Ambient ionization techniques are new mass spectrometric techniques that allow direct analysis of samples with no or little sample preparation. Can these techniques facilitate or even eliminate sample preparation for mass spectrometric analysis of proteins? Apart from sample preparation, do these techniques offer any new features for characterization of proteins as compared with conventional ESI or MALDI? Recent advances in characterization of proteins by ambient mass spectrometry are summarized and commented in this article.
蛋白质在生命系统中起着重要的作用，是许多基础研究和应用研究项目的主题。随着 20 世纪 80 年代后期电喷雾电离和基质辅助激光解吸/电离技术在生物大分子分析中的引入，质谱已经成为蛋白质表征的重要工具。然而，通过这些质谱技术对原始样品中的蛋白质进行表征通常需要广泛的样品预处理。大气离子化技术是一种新的质谱技术，它可以直接分析未经或很少预处理的样品。这些技术是否可以促进甚至消除蛋白质的质谱分析的样品制备？与传统的 ESI 或 MALDI 相比，除了样品制备之外，这些技术在蛋白质的表征方面是否提供了任何新的特征？本文对大气质谱在蛋白质表征方面的最新进展进行了总结和评论。
