Laboratoire Eau, Molécules actives, Macromolécules, Activité, AgroSup Dijon, Université de Bourgogne, Dijon, France.
Lett Appl Microbiol. 2011 Nov;53(5):558-64. doi: 10.1111/j.1472-765X.2011.03149.x. Epub 2011 Sep 29.
This study aimed to investigate the effect of copper sulfate (from 0 to 8 mmol kg(-1)) on radial growth rate and lag time of two moulds responsible for vine grapes spoilage: Penicillium expansum strain 25·03 and Botrytis cinerea, strains BC1 and BC2.
A new model was developed to describe tailing and shoulders in the inhibition curves. Because of tailing, the minimum inhibitory concentration (MIC), was not defined as the concentration at which no growth was observed, but as the concentration at which the lag time was infinite. The concentrations at which μ = μ(opt)/2, (Cu50), were in the range of 2·2-2·6 mmol kg(-1). Radial growth rate of P. expansum and the reciprocal of the lag time were linearly correlated (r = 0·84). In contrast, in the range 0-4 mmol kg(-1), an inhibition of growth of B. cinerea was observed whereas germination remained unaffected (i.e. the lag time was constant). In the range 4-8 mmol kg(-1) , the radial growth rate of B. cinerea was almost constant (c. 1 mm day(-1)), but germination was inhibited (i.e. the lag time was increased).
The MIC values were 4·7 mmol kg(-1) for P. expansum, 8·2 and 7·3 mmol kg(-1) for B. cinerea strain BC1 and BC2, respectively, demonstrating that some isolates of these moulds are resistant to copper.
Copper concentrations at 4 mmol kg(-1) would be sufficient to control the development of these isolates, but the toxicity of copper should be extended to other isolates and evaluated in vineyards.
本研究旨在探讨硫酸铜（0 至 8mmolkg(-1)）对两种导致葡萄腐烂的霉菌：扩展青霉菌株 25·03 和葡萄孢菌菌株 BC1 和 BC2 的径向生长率和滞后时间的影响。
开发了一种新的模型来描述抑制曲线中的尾部和肩部。由于尾部，最小抑制浓度（MIC）不是定义为没有观察到生长的浓度，而是定义为滞后时间无限长的浓度。μ=μ(opt)/2 时的浓度（Cu50）在 2.2-2.6mmolkg(-1)范围内。扩展青霉的径向生长率与滞后时间的倒数呈线性相关（r=0.84）。相比之下，在 0-4mmolkg(-1)范围内，观察到对葡萄孢菌生长的抑制，但萌发不受影响（即滞后时间保持不变）。在 4-8mmolkg(-1)范围内，葡萄孢菌的径向生长率几乎保持不变（约 1mm 天(-1)），但萌发受到抑制（即滞后时间增加）。
对于扩展青霉，MIC 值为 4.7mmolkg(-1)，对于葡萄孢菌菌株 BC1 和 BC2，分别为 8.2 和 7.3mmolkg(-1)，表明这些霉菌的一些分离物对铜具有抗性。
4mmolkg(-1)的铜浓度足以控制这些分离物的发展，但铜的毒性应扩展到其他分离物，并在葡萄园进行评估。
