Hirata Y, Orth D N
J Clin Endocrinol Metab. 1979 Apr;48(4):667-72. doi: 10.1210/jcem-48-4-667.
Human epidermal growth factor (hEGF), which stimulates the growth of a variety of tissues, was first isolated from mouse submandibular glands, but is also excreted in large amounts (about 50 micrograms/day) in human urine and is probably identical to human beta-urogastrone (hUG), a potent inhibitor of stimulated gastric acid secretion. However, the primary tissue source of hEGF/hUG is as yet unknown. The hEGF/hUG in homogenates of human salivary glands and a wide variety of other endocrine and nonendocrine tissues was extracted by Amberlite CG-50 cation exchange chromatography and immune affinity chromatography using the immunoglobulin fraction of rabbit anti-hEGF serum covalently bound to agarose. The extracts were subjected to homologous hEGF RIA. Immunoreactive hEGF was found in extracts of adult submandibular gland, thyroid gland, duodenum, jejunum, and kidney, but not in several fetal tissues. The tissue immunoreactive hEGF was similar to standard hEGF in terms of immunoreactivity and elution from Sephadex G-50 Fine resin, but its concentrations were very low (1.3-5.5 ng/g wet tissue). Thus, it is not certain that these tissues represent the only source of the large amounts of hEGF/hUG that appear to be filtered by the kidneys each day.
人表皮生长因子（hEGF）可刺激多种组织生长，最初是从小鼠颌下腺中分离出来的，但在人尿中也大量排泄（约50微克/天），并且可能与人β-尿抑胃素（hUG）相同，后者是胃酸分泌的有效抑制剂。然而，hEGF/hUG的主要组织来源尚不清楚。采用共价结合到琼脂糖上的兔抗hEGF血清免疫球蛋白部分，通过Amberlite CG-50阳离子交换色谱法和免疫亲和色谱法从人唾液腺及多种其他内分泌和非内分泌组织的匀浆中提取hEGF/hUG。提取物进行同源hEGF放射免疫分析。在成人颌下腺、甲状腺、十二指肠、空肠和肾脏的提取物中发现了免疫反应性hEGF，但在几种胎儿组织中未发现。组织免疫反应性hEGF在免疫反应性和从Sephadex G-50 Fine树脂上洗脱方面与标准hEGF相似，但其浓度非常低（1.3 - 5.5纳克/克湿组织）。因此，尚不能确定这些组织是否代表每天似乎被肾脏滤过的大量hEGF/hUG的唯一来源。
