Watanabe H, Ohashi Y, Kinoshita S, Manabe R, Ohshiden K
Department of Ophthalmology, Osaka University Medical School, Japan.
Graefes Arch Clin Exp Ophthalmol. 1993 Apr;231(4):228-32. doi: 10.1007/BF00918846.
Using a sensitive radioimmunoassay (RIA) specific for rat epidermal growth factor (rEGF), we investigated the presence of rEGF in a variety of rat ocular and periocular tissues. Immunoreactive rEGF (IR-rEGF) was present in tear fluids (25.5 +/- 5.8 ng/ml), exorbital lacrimal gland (6.73 ng/g wet weight), intraorbital lacrimal gland (2.80 ng/g wet weight), Harderian gland (1.90 ng/g wet weight), and conjunctiva (0.16 ng/g wet weight). EGF was not detectable in aqueous humor, cornea, iris and ciliary body, lens, or the posterior part of the globe (retina, choroid, and vitreous body). Gel exclusion chromatography and reverse-phase high-performance liquid chromatography revealed that IR-rEGF in the above ocular fluids and tissues was indistinguishable from standard rEGF. Using enzyme-linked immunohistochemistry, rEGF was demonstrated to be localized in the duct epithelial cells of lacrimal glands. These findings reveal that EGF is preferentially localized in the ocular surface and lacrimal apparatus.
利用一种对大鼠表皮生长因子（rEGF）具有特异性的灵敏放射免疫分析法（RIA），我们研究了多种大鼠眼及眼周组织中rEGF的存在情况。免疫反应性rEGF（IR-rEGF）存在于泪液（25.5±5.8纳克/毫升）、眶外泪腺（6.73纳克/克湿重）、眶内泪腺（2.80纳克/克湿重）、哈德氏腺（1.90纳克/克湿重）和结膜（0.16纳克/克湿重）中。在房水、角膜、虹膜和睫状体、晶状体或眼球后部（视网膜、脉络膜和玻璃体）中未检测到EGF。凝胶排阻色谱法和反相高效液相色谱法显示，上述眼液和组织中的IR-rEGF与标准rEGF无法区分。利用酶联免疫组织化学法，证明rEGF定位于泪腺的导管上皮细胞中。这些发现表明，EGF优先定位于眼表和泪器。
