Hirata Y, Uchihashi M, Nakajima M, Fujita T, Matsukura S
J Clin Endocrinol Metab. 1982 Jun;54(6):1242-5. doi: 10.1210/jcem-54-6-1242.
Human epidermal growth factor (hEGF), which stimulates the growth of a variety of cells in culture, has recently been isolated from human urine. In the present study, we identified significant amounts of immunoreactive (IR) hEGF (mean +/- SE, 2.3 +/- 0.09 ng/ml; n = 3) in human pancreatic juice. The IR-hEGF materials were immunologically indistinguishable from standard hEGF, although they were only 5% as active in receptor binding to human placental membrane as in RIA. Gel exclusion chromatography of the pancreatic juice under neutral and acidic conditions revealed three distinct IR-hEGF components with different molecular sizes. Incubation of 125I-labeled hEGF with either the pancreatic juice or the high molecular weight component(s) yielded no aggregation or degradation products. These data suggest that fully immunoreactive but much less bioactive hEGF-like substances which are heterogeneous in size are secreted into human pancreatic juice. Their tissue(s) of origin and physiological functions, if any, remain to be elucidated.
人表皮生长因子（hEGF）能刺激多种培养细胞的生长，最近已从人尿中分离出来。在本研究中，我们在人胰液中鉴定出了大量的免疫反应性（IR）hEGF（平均值±标准误，2.3±0.09 ng/ml；n = 3）。尽管胰液中的IR - hEGF物质与标准hEGF在免疫方面无法区分，但它们与人类胎盘膜受体结合的活性仅为放射免疫分析（RIA）中的5%。在中性和酸性条件下对胰液进行凝胶排阻色谱分析，发现了三种不同分子大小的明显的IR - hEGF组分。用125I标记的hEGF与胰液或高分子量组分孵育，未产生聚集或降解产物。这些数据表明，大小各异的具有完全免疫反应性但生物活性低得多的类hEGF物质被分泌到人胰液中。它们的起源组织和生理功能（如果有的话）仍有待阐明。
