Division of Infectious Disease, Children's Hospital, Boston, Massachusetts, United States of America.
PLoS Pathog. 2011 Aug;7(8):e1002210. doi: 10.1371/journal.ppat.1002210. Epub 2011 Aug 25.
While the exopolysaccharide component of the biofilm matrix has been intensively studied, much less is known about matrix-associated proteins. To better understand the role of these proteins, we undertook a proteomic analysis of the V. cholerae biofilm matrix. Here we show that the two matrix-associated proteins, Bap1 and RbmA, perform distinct roles in the biofilm matrix. RbmA strengthens intercellular attachments. In contrast, Bap1 is concentrated on surfaces where it serves to anchor the biofilm and recruit cells not yet committed to the sessile lifestyle. This is the first example of a biofilm-derived, communally synthesized conditioning film that stabilizes the association of multilayer biofilms with a surface and facilitates recruitment of planktonic bystanders to the substratum. These studies define a novel paradigm for spatial and functional differentiation of proteins in the biofilm matrix and provide evidence for bacterial cooperation in maintenance and expansion of the multilayer biofilm.
虽然生物膜基质的胞外多糖成分已经得到了深入研究，但对于基质相关蛋白的了解却要少得多。为了更好地了解这些蛋白质的作用，我们对霍乱弧菌生物膜基质进行了蛋白质组学分析。在这里，我们表明两种基质相关蛋白，Bap1 和 RbmA，在生物膜基质中发挥不同的作用。RbmA 增强细胞间的附着。相比之下，Bap1 集中在表面，用于固定生物膜并招募尚未适应定殖生活方式的细胞。这是生物膜衍生的、共同合成的调理膜的第一个例子，它稳定了多层生物膜与表面的关联，并促进浮游生物旁观者向基质的招募。这些研究定义了生物膜基质中蛋白质空间和功能分化的新范例，并为细菌在维持和扩展多层生物膜方面的合作提供了证据。
