使用在简并氨基酸密码子的模糊位置含有脱氧肌苷的混合引物对大肠杆菌基因进行PCR扩增。 - Suppr

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超能文献

PCR amplification of an Escherichia coli gene using mixed primers containing deoxyinosine at ambiguous positions in degenerate amino acid codons.

作者信息

Patil R V, Dekker E E

机构信息

Department of Biological Chemistry, University of Michigan, Ann Arbor 48109-0606.

出版信息

Nucleic Acids Res. 1990 May 25;18(10):3080. doi: 10.1093/nar/18.10.3080.

DOI:10.1093/nar/18.10.3080

PMID:2190190

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC330866/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/772e/330866/633a5a2bff6c/nar00194-0225-a.jpg

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PCR amplification of an Escherichia coli gene using mixed primers containing deoxyinosine at ambiguous positions in degenerate amino acid codons.使用在简并氨基酸密码子的模糊位置含有脱氧肌苷的混合引物对大肠杆菌基因进行PCR扩增。

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本文引用的文献

The complete amino acid sequence and identification of the active-site arginine peptide of Escherichia coli 2-keto-4-hydroxyglutarate aldolase.大肠杆菌2-酮-4-羟基戊二酸醛缩酶的完整氨基酸序列及活性位点精氨酸肽的鉴定。

J Biol Chem. 1988 Aug 25;263(24):11683-91.

Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.用于镰状细胞贫血诊断的β-珠蛋白基因组序列的酶促扩增及限制性酶切位点分析。

Science. 1985 Dec 20;230(4732):1350-4. doi: 10.1126/science.2999980.

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