大肠杆菌N-乙酰神经氨酸裂解酶基因的遗传与分子分析
Genetic and molecular analyses of Escherichia coli N-acetylneuraminate lyase gene.
作者信息
Kawakami B, Kudo T, Narahashi Y, Horikoshi K
出版信息
J Bacteriol. 1986 Jul;167(1):404-6. doi: 10.1128/jb.167.1.404-406.1986.
Abstract
Two plasmids containing the N-acetylneuraminate lyase (NALase) gene (nanA) of Escherichia coli, pNL1 and pNL4, were constructed. Immunoprecipitation analysis indicated that the 35,000-dalton protein encoded in pNL4 was NALase. The synthesis of NALase in E. coli carrying these plasmids was constitutive.
摘要
构建了两个含有大肠杆菌N-乙酰神经氨酸裂解酶（NALase）基因（nanA）的质粒，即pNL1和pNL4。免疫沉淀分析表明，pNL4中编码的35000道尔顿蛋白是NALase。携带这些质粒的大肠杆菌中NALase的合成是组成型的。
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