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细菌转铁蛋白和乳铁蛋白受体蛋白基因的快速鉴定与克隆

Rapid identification and cloning of bacterial transferrin and lactoferrin receptor protein genes.

作者信息

Ogunnariwo J A, Schryvers A B

机构信息

Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada.

出版信息

J Bacteriol. 1996 Dec;178(24):7326-8. doi: 10.1128/jb.178.24.7326-7328.1996.

DOI:10.1128/jb.178.24.7326-7328.1996
PMID:8955421
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC178652/
Abstract

The sequences of genes encoding the transferrin and lactoferrin receptor proteins from several bacterial species were analyzed for areas of identity in the predicted protein sequences. Degenerate oligonucleotide primers were designed and tested for their ability to amplify portions of the receptor genes. Primer pairs capable of amplifying products of the tbpA/lbpA or tbpB/lbpB genes from all species possessing these receptors were identified.

摘要

对几种细菌物种中编码转铁蛋白和乳铁蛋白受体蛋白的基因序列进行了分析，以寻找预测蛋白质序列中的相同区域。设计了简并寡核苷酸引物，并测试了它们扩增受体基因部分序列的能力。鉴定出了能够从所有拥有这些受体的物种中扩增出tbpA/lbpA或tbpB/lbpB基因产物的引物对。

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本文引用的文献

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Bacterial transferrin and lactoferrin receptors.细菌转铁蛋白和乳铁蛋白受体。
Trends Microbiol. 1996 May;4(5):185-91. doi: 10.1016/0966-842x(96)10025-1.
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Transferrin-binding proteins isolated from Neisseria meningitidis elicit protective and bactericidal antibodies in laboratory animals.从脑膜炎奈瑟菌中分离出的转铁蛋白结合蛋白可在实验动物中引发保护性和杀菌性抗体。
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Gonococcal transferrin-binding protein 2 facilitates but is not essential for transferrin utilization.淋球菌转铁蛋白结合蛋白2有助于转铁蛋白的利用，但并非必不可少。
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Identification and characterization of genes encoding the human transferrin-binding proteins from Haemophilus influenzae.流感嗜血杆菌中人转铁蛋白结合蛋白编码基因的鉴定与特性分析。
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Evaluation of transferrin-binding protein 2 within the transferrin-binding protein complex as a potential antigen for future meningococcal vaccines.评估转铁蛋白结合蛋白复合物中的转铁蛋白结合蛋白2作为未来脑膜炎球菌疫苗潜在抗原的情况。
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Molecular characterization of the structural gene for the lactoferrin receptor of the meningococcal strain H44/76.脑膜炎球菌菌株H44/76乳铁蛋白受体结构基因的分子特征分析
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Microbiology (Reading). 1995 Oct;141 ( Pt 10):2405-16. doi: 10.1099/13500872-141-10-2405.
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PCR amplification of an Escherichia coli gene using mixed primers containing deoxyinosine at ambiguous positions in degenerate amino acid codons.使用在简并氨基酸密码子的模糊位置含有脱氧肌苷的混合引物对大肠杆菌基因进行PCR扩增。
Nucleic Acids Res. 1990 May 25;18(10):3080. doi: 10.1093/nar/18.10.3080.
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Characterization of two genes encoding distinct transferrin-binding proteins in different Actinobacillus pleuropneumoniae isolates.不同胸膜肺炎放线杆菌分离株中两个编码不同转铁蛋白结合蛋白的基因的特性分析。
Infect Immun. 1992 Aug;60(8):3253-61. doi: 10.1128/iai.60.8.3253-3261.1992.
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Immunization of pigs against Actinobacillus pleuropneumoniae with two recombinant protein preparations.用两种重组蛋白制剂对猪进行胸膜肺炎放线杆菌免疫接种。
Vaccine. 1992;10(8):512-8. doi: 10.1016/0264-410x(92)90349-o.