Zhang C Y, Dong L, Wang J, Chen S L
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Pharmazie. 2011 Aug;66(8):553-9.
A rapid, sensitive and reliable method based on ultra performance liquid chromatography coupled with a photodiode array detector (UPLC-PAD) was developed for both the quantitative analysis of ten bioactive ginsenosides and a chemical-fingerprint analysis. The chromatography was performed on an ACQUITY UPLC BEH C18 column using a gradient elution with acetonitrile/water as the mobile phase. To compare the UPLC fingerprints and to evaluate their quality, chemometric methods including similarity analysis (SA) and hierarchical-clustering analysis (HCA) were implemented when classifying the Panacis Quinquefolii Radix samples. The Panacis Quinquefolii Radix samples were successfully grouped in accordance with their geographic origins.
建立了一种基于超高效液相色谱-光电二极管阵列检测器（UPLC-PAD）的快速、灵敏且可靠的方法，用于十种生物活性人参皂苷的定量分析和化学指纹图谱分析。色谱分析在ACQUITY UPLC BEH C18柱上进行，以乙腈/水为流动相进行梯度洗脱。为比较UPLC指纹图谱并评估其质量，在对西洋参样品进行分类时采用了包括相似度分析（SA）和层次聚类分析（HCA）在内的化学计量学方法。西洋参样品成功地根据其地理来源进行了分组。
