Huang Yan-Fei, Sun Mei, Xu Yun-Zhang, Wang Jing-Xia, Zhao Xiao-Yan, Chen Ke-Li, Huang Bi-Sheng, Liu Yuan
Zhongguo Zhong Yao Za Zhi. 2014 Oct;39(20):3950-4.
The influence on 10 kinds of ginsensides of different processed methods of Panacis Quinquefolii Radix was discussed. White Panacis Quinquefolii Radix (sliced and dried at -80 °C), red Panacis Quinquefolii Radix( steamed, sliced and dried at -80 °C) and commercial Radix Panacis Quinquefolii (dried by electric blast air) processed by different methods. HPLC-PDA-ESI- MS method was established before by our team. Ten kinds of ginsenosides of them were determined. The content of total ginsenosides were as follow: commercial Panacis Quinquefolii Radix > white Panacis Quinquefolii Radix > red Panacis Quinquefolii Radix. Compared with white Panacis Quinquefolii Radix, the content of Re, Rc, Rb3 and Rb2 of Red Radix Panacis Quinquefolii decreased but increased that of Rg,, Rb1. Both Rg2 and Rg, were not found in white Panacis Quinquefolii Radix and commercial Panacis Quinquefolii Radix by PDA detector, and low response in ESI-MS, while red Panacis Quinquefolii Radix was to the high content that of 0. 027% and 0.040 1%. The constituent of RA0 of red Panacis Quinquefolii Radix was higher than the other two. After Panacis Quinquefolii Radix processed, the kind and content of ginsensides were significantly changed. The constituent of some kinds of ginsensides was increased and some decreased. Rf was not found in all Panacis Quinquefolii Radix samples which were consistent with the former documents.
探讨了不同加工方法对西洋参10种人参皂苷的影响。西洋参经不同方法加工得到生晒参（-80℃切片干燥）、红参（蒸制后-80℃切片干燥）和市售西洋参（电热鼓风干燥）。本团队之前建立了HPLC-PDA-ESI-MS方法，测定了其中的10种人参皂苷。总人参皂苷含量如下：市售西洋参＞生晒参＞红参。与生晒参相比，红参中Re、Rc、Rb3和Rb2的含量降低，但Rg1、Rb1的含量增加。通过PDA检测器在生晒参和市售西洋参中均未检测到Rg2和Rg3，ESI-MS响应较低，而红参中Rg2和Rg3的含量较高，分别为0.027%和0.0401%。红参中RA0的成分高于其他两者。西洋参加工后，人参皂苷的种类和含量发生了显著变化，部分人参皂苷成分增加，部分减少。所有西洋参样品中均未检测到Rf，这与之前的文献一致。
