西洋参高效液相色谱-紫外-蒸发光散射特征图谱及化学模式识别
[HPLC-UV-ELSD characteristic figure and chemical pattern recognition of Panacis Quinquefolii Radix].
作者信息
Dong Liang, Zhang Cui-ying, Chen Shi-lin
机构信息
Henan University of Traditional Chinese Medicine, Zhengzhou 450003, China.
出版信息
Yao Xue Xue Bao. 2011 Feb;46(2):198-202.
The paper is to report the establishment of a method of characteristic figure analysis for the quality control of Panacis Quinquefolii Radix. Application of HPLC-UV-ELSD techniques was connected in series and applied. The separation was carried out on the Agilent Extend-C18 (250 mm x 4.6 mm, 5 microm) column. The mobile phase consisted of water and acetonitrile with gradient elution. The flow rate was 1.0 mL x min(-1) and the wavelength of measurement was 203 nm. The temperature of drift tube was maintained at 106.5 degrees C and the flow rate of air was set at 2.9 L x min(-1). Twenty batches of the Panacis Quinquefolii Radix were determined. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) were applied to study on the HPLC characteristic figure and chemical pattern recognition. The HPLC-UV and HPLC-ELSD characteristic figure of Panacis Quinquefolii Radix was developed, the ginsenosides Rg1, Re, Rb1, Rc, Rb2, Rb3, Rd and the pseudoginsenoside F11 were identified. This method is accurate and reliable, and it can be used to control the quality of the Panacis Quinquefolii Radix.
本文报道西洋参质量控制特征图谱分析方法的建立。串联应用HPLC - UV - ELSD技术并进行了研究。采用Agilent Extend - C18（250 mm×4.6 mm，5μm）色谱柱进行分离。流动相由水和乙腈组成，梯度洗脱。流速为1.0 mL·min⁻¹，检测波长为203 nm。漂移管温度保持在106.5℃，空气流速设定为2.9 L·min⁻¹。测定了20批西洋参。应用聚类分析（HCA）和主成分分析（PCA）对西洋参HPLC特征图谱及化学模式识别进行研究。建立了西洋参HPLC - UV和HPLC - ELSD特征图谱，鉴定了人参皂苷Rg1、Re、Rb1、Rc、Rb2、Rb3、Rd及拟人参皂苷F11。该方法准确可靠，可用于西洋参的质量控制。
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