Oliveira Vânia, Bártolo Inês, Borrego Pedro, Rocha Cheila, Valadas Emília, Barreto Jorge, Almeida Elsa, Antunes Francisco, Taveira Nuno
Laboratório de Hemato-Oncologia, Instituto Português de Oncologia, Lisbon, Portugal.
AIDS Res Hum Retroviruses. 2012 May;28(5):510-22. doi: 10.1089/AID.2011.0174. Epub 2011 Sep 19.
Our aim was to characterize for the first time the genetic diversity of HIV in Cape Verde Islands as well as the drug resistance profiles in treated and untreated patients. Blood specimens were collected from 41 HIV-1 and 14 HIV-2 patients living in Santiago Island. Half of the patients were on antiretroviral treatment (ART). Pol and env gene sequences were obtained using in-house methods. Phylogenetic analysis was used for viral subtyping and the Stanford Algorithm was used for resistance genotyping. For HIV-1, the amplification of pol and env was possible in 27 patients (66%). HIV-1 patients were infected with subtypes G (13, 48%), B (2, 7%), F1 (2, 7%), and CRF02_AG (2, 7%), and complex recombinant forms including a new C/G variant (n=8, 30%). Drug resistance mutations were detected in the PR and RT of three (10%) treated patients. M41L and K103N transmitted drug resistance mutations were found in 2 of 17 (12%) untreated patients. All 14 HIV-2 isolates belonged to group A. The origin of 12 strains was impossible to determine whereas two strains were closely related to the historic ROD strain. In conclusion, in Cape Verde there is a long-standing HIV-2 epidemic rooted in ROD-like strains and a more recent epidemic of unknown origin. The HIV-1 epidemic is caused by multiple subtypes and complex recombinant forms. Drug resistance HIV-1 strains are present at moderate levels in both treated and untreated patients. Close surveillance in these two populations is crucial to prevent further transmission of drug-resistant strains.
我们的目标是首次描述佛得角群岛艾滋病毒的基因多样性以及接受治疗和未接受治疗患者的耐药性概况。从圣地亚哥岛的41名HIV-1患者和14名HIV-2患者中采集了血液样本。一半的患者正在接受抗逆转录病毒治疗（ART）。使用内部方法获得了Pol和env基因序列。系统发育分析用于病毒亚型分型，斯坦福算法用于耐药基因分型。对于HIV-1，27名患者（66%）的Pol和env能够扩增。HIV-1患者感染的亚型为G型（13例，48%）、B型（2例，7%）、F1型（2例，7%）和CRF02_AG型（2例，7%），以及包括一种新的C/G变体在内的复杂重组形式（n = 8例，30%）。在3名（10%）接受治疗的患者的蛋白酶（PR）和逆转录酶（RT）中检测到耐药突变。在17名未接受治疗的患者中的2名（12%）发现了M41L和K103N传播性耐药突变。所有14株HIV-2分离株均属于A组。12株病毒的起源无法确定，而有2株与历史ROD株密切相关。总之，在佛得角，存在着一种源于类似ROD株的长期HIV-2流行以及一种起源不明的较新流行。HIV-1流行是由多种亚型和复杂重组形式引起的。耐药HIV-1毒株在接受治疗和未接受治疗的患者中均以中等水平存在。对这两个人群进行密切监测对于防止耐药毒株的进一步传播至关重要。
