Department of Bioengineering, National University of Singapore, Singapore.
Tissue Eng Part B Rev. 2012 Apr;18(2):77-87. doi: 10.1089/ten.TEB.2011.0390. Epub 2011 Dec 14.
In the past decade, considerable effort has been made to construct biomimetic scaffolds from electrospun nanofibers for engineering different tissues. However, one of the major concerns with electrospun nanofibrous scaffolds is that the densely arranged architecture of fibers and small pores or voids between fibers hinder efficient cellular infiltration or prevent three dimensional (3D) cellular integration with host tissue in vivo after implantation. To overcome this problem, many concepts or strategies applicable during the electrospinning or post-electrospinning procedures have been proposed to enlarge pore size of electrospun scaffolds. This article addresses the issues of pore geometry and cellular infiltration of electrospun scaffolds, and first reviews the fabrication solutions/approaches applied to achieve larger micropores in electrospun mats. The evidence and potential for fostering cellular infiltration using these improved porous scaffolds are then discussed. Finally, it is hoped that this will enable us to better exploit viable technologies or develop new ones for constructing ideal nanofibrous architecture for fulfilling specific tissue engineering needs.
在过去的十年中，人们已经做出了相当大的努力，通过静电纺丝技术从纳米纤维中构建仿生支架，用于工程不同的组织。然而，静电纺丝纳米纤维支架的一个主要问题是，纤维的密集排列结构和纤维之间的小孔或空隙阻碍了有效的细胞渗透，或者在植入后防止了三维（3D）细胞与宿主组织的整合。为了解决这个问题，在静电纺丝或静电纺丝后处理过程中提出了许多概念或策略，以增大静电纺丝支架的孔径。本文讨论了静电纺丝支架的孔径几何形状和细胞渗透问题，首先综述了实现静电纺丝毡中大微孔的制造解决方案/方法。然后讨论了使用这些改进的多孔支架促进细胞渗透的证据和潜力。最后，希望这将使我们能够更好地利用可行的技术或开发新的技术，构建理想的纳米纤维结构，以满足特定的组织工程需求。
