Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
Tissue Eng Part A. 2011 Nov;17(21-22):2695-702. doi: 10.1089/ten.TEA.2010.0709. Epub 2011 Jul 28.
A significant problem that affects tissue-engineered electrospun nanofibrous scaffolds is poor infiltration of cells into the three-dimensional (3D) structure. Physical manipulation can enhance cellular infiltration into electrospun scaffolds. The porosity of electrospun nanofibers was highly enlarged by ultrasonication in an aqueous solution. The porosity and related property changes on a series of nanofibers were observed to be dependent on ultrasonication time and energy. To evaluate cell infiltration into the scaffold, fibroblasts were seeded onto these nanofibers and cultured for different lengths of time. The penetration levels of these cells into the scaffold were monitored using confocal lazer scanning microscopy. The cell infiltration potential was greatly increased with regard to an increase in pore size and porosity. These 3D nanofibrous scaffolds fabricated by an ultrasonication process allowed cells to infiltrate easily into the scaffold. This approach shows great promise for design of cell permeable nanofibrous scaffolds for tissue-engineering applications.
一个影响组织工程电纺纳米纤维支架的重大问题是细胞难以渗透到三维（3D）结构中。物理处理可以增强细胞对电纺支架的渗透。在水溶液中进行超声处理可以极大地增加电纺纳米纤维的孔隙率。观察到一系列纳米纤维的孔隙率和相关性能变化取决于超声处理时间和能量。为了评估细胞渗透到支架中，将成纤维细胞接种到这些纳米纤维上并培养不同的时间。使用共聚焦激光扫描显微镜监测这些细胞渗透到支架中的水平。细胞渗透的潜力随着孔径和孔隙率的增加而大大增加。通过超声处理制备的这些 3D 纳米纤维支架允许细胞容易地渗透到支架中。这种方法为用于组织工程应用的细胞可渗透纳米纤维支架的设计提供了很大的希望。
