正常人血浆中免疫反应性β-内啡肽的存在：甲吡酮给药后β-内啡肽与促肾上腺皮质激素的伴随释放。
Presence of immunoreactive beta-endorphin in normal human plasma: a concomitant release of beta-endorphin with adrenocorticotropin after metyrapone administration.
作者信息
Nakao K, Nakai Y, Oki S, Horii K, Imura H
出版信息
J Clin Invest. 1978 Dec;62(6):1395-8. doi: 10.1172/JCI109261.
Abstract
To elucidate whether or not beta-endorphin exists in plasma of normal subjects, plasma extracts obtained before and after metyrapone administration were subjected to gel exclusion chromatography, and fractions obtained were assayed by a sensitive radioimmunoassay for beta-endorphin. The basal plasma level of beta-endorphin was 5.8 +/- 1.1 pg/ml (mean +/- SE, n = 5), which rose significantly to the level of 48.9 +/- 3.8 pg/ml after a single oral dose (30 mg/kg of body wt) of metyrapone administration (P less than 0.001). Plasma ACTH levels also increased from the mean basal level of 73 +/- 4 pg/ml to 269 +/- 41 pg/ml after metyrapone administration. These results indicate that beta-endorphin, distinct from beta-lipotropin, exists in normal human plasma and that it is released from the pituitary concomitantly with ACTH.
摘要
为了阐明正常受试者血浆中是否存在β-内啡肽，对服用甲吡酮前后获得的血浆提取物进行凝胶排阻色谱分析，并用灵敏的β-内啡肽放射免疫分析法对所得组分进行检测。β-内啡肽的基础血浆水平为5.8±1.1 pg/ml（平均值±标准误，n = 5），单次口服剂量（30 mg/kg体重）的甲吡酮后，其水平显著升高至48.9±3.8 pg/ml（P＜0.001）。服用甲吡酮后，血浆促肾上腺皮质激素（ACTH）水平也从平均基础水平73±4 pg/ml升高至269±41 pg/ml。这些结果表明，与β-促脂素不同，β-内啡肽存在于正常人血浆中，并且它与ACTH一起从垂体释放。
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