经 GATC 序列上下文改变修饰的噬菌体 ΦX174 的突变率。

Mutation rate of bacteriophage ΦX174 modified through changes in GATC sequence context.

机构信息

Instituto Cavanilles de Biodiversidad y Biología Evolutiva and Departamento de Genética, Universitat de València, Spain.

出版信息

Infect Genet Evol. 2011 Oct;11(7):1820-2. doi: 10.1016/j.meegid.2011.08.024. Epub 2011 Aug 31.

DOI:10.1016/j.meegid.2011.08.024

PMID:21903178

Abstract

Bacteriophage ΦX174 has a relatively high mutation rate of 10⁻⁶ substitutions per nucleotide per strand copying. A thirty-fold reduction in the mutation rate was achieved by introducing seven GATC sequences in its genome. This motif allows for methyl-directed mismatch repair and is strongly avoided in nature by ΦX174 and other phages.

摘要

噬菌体 ΦX174 的突变率相对较高，每链每核苷酸复制时有 10⁻⁶ 的替换率。通过在其基因组中引入七个 GATC 序列，突变率降低了三十倍。该基序允许甲基化指导的错配修复，并且被 ΦX174 和其他噬菌体强烈回避。