Stem Cell Facility, All India Institute of Medical Sciences, N. Delhi,
Int J Cardiol. 2013 Feb 10;163(1):93-9. doi: 10.1016/j.ijcard.2011.08.003. Epub 2011 Sep 7.
The majority of the protocols for cardiomyocyte differentiation of MSC use 5-azacytidine as an inducer. As transforming growth factor β1 and 5-azacytidine share similar target signaling pathways, we examined whether transforming growth factor β1 can play a role in cardiac differentiation process in human mesenchymal stem cell of bone marrow origin.
The differentiation protocol involving transforming growth factor β1 was compared with that of 5-azacytidine in these cells. The two differentiation regimes were compared using reverse transcriptase PCR, flow cytometry, and quantitative PCR.
We observed that in both cases, acquired morphological features were similar. Protein and gene expression assays also indicated similar cardiac marker expression profile in both the differentiation conditions. Furthermore, transforming growth factor β1 and 5-azacytidine allowed the acquisition of comparable levels of cardiac cell like molecular characteristic as attested by evaluation of myosin light chain-2v expression.
In conclusion, we demonstrate that transforming growth factor β1 can play a similar role in cardiac differentiation process of human bone marrow mesenchymal stem cells.
大多数用于 MSC 心肌细胞分化的方案都使用 5-氮杂胞苷作为诱导剂。由于转化生长因子 β1 和 5-氮杂胞苷具有相似的靶信号通路，我们研究了转化生长因子 β1 是否可以在骨髓来源的人间质干细胞的心脏分化过程中发挥作用。
将涉及转化生长因子 β1 的分化方案与该细胞中的 5-氮杂胞苷进行比较。使用逆转录 PCR、流式细胞术和定量 PCR 比较两种分化方案。
我们观察到，在两种情况下，获得的形态特征相似。蛋白质和基因表达分析也表明，两种分化条件下的心脏标记物表达谱相似。此外，转化生长因子 β1 和 5-氮杂胞苷允许获得类似水平的心肌样分子特征，这可以通过评估肌球蛋白轻链-2v 的表达来证明。
总之，我们证明转化生长因子 β1 可以在人骨髓间充质干细胞的心脏分化过程中发挥类似的作用。
