Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India.
Bioresour Technol. 2012 Jan;104:459-65. doi: 10.1016/j.biortech.2011.10.043. Epub 2011 Oct 20.
Delignification of paddy straw with the white-rot fungus, Trametes hirsuta under solid state fermentation, for enhanced sugar recovery by enzymatic saccharification was studied. T. hirsuta MTCC136 showed high ligninase and low cellulase activities. Solid state fermentation of paddy straw with T. hirsuta enhanced carbohydrate content by 11.1% within 10 days of incubation. Alkali extracts of Trametes pretreated paddy straw showed high absorbance at 205 nm indicating high lignin break down. The amount of value-added lignin recovered from the Trametes pretreated paddy straw was much higher than controls. Enzymatic hydrolysis of the Trametes pretreated paddy straw yielded much higher sugars than controls and yields increased till 120 h of incubation. Saccharification efficiency of the biologically pretreated paddy straw with Accelerase®1500 was 52.69% within 72 h and was higher than controls. Thus, the study brings out the delignification potential of T. hirsuta for pretreatment of lignocellulosic substrate and facilitating efficient enzymatic digestibility of cellulose.
采用白腐菌糙皮侧耳（Trametes hirsuta）进行固态发酵对稻草进行脱木质素处理，以提高酶解糖化过程中的糖回收率，本研究对此进行了探讨。T. hirsuta MTCC136 表现出较高的木质素酶和较低的纤维素酶活性。在 10 天的培养期内，糙皮侧耳固态发酵使稻草的碳水化合物含量增加了 11.1%。预处理后的稻草碱提取物在 205nm 处具有较高的吸光度，表明木质素分解程度较高。从糙皮侧耳预处理后的稻草中回收的附加值木质素的量远高于对照。与对照相比，预处理后的稻草经酶解后可产生更多的糖，且在 120 小时的孵育期内产量仍在增加。用 Accelerase®1500 对生物预处理的稻草进行糖化，72 小时内的糖化效率为 52.69%，高于对照。因此，该研究揭示了糙皮侧耳对木质纤维素基质进行预处理的脱木质素潜力，有利于纤维素的高效酶解。
