Department of Neurology, Henry Ford Hospital, E&R 3056, 2799 West Grand Boulevard, Detroit, MI 48202, USA.
Stroke. 2011 Dec;42(12):3537-41. doi: 10.1161/STROKEAHA.111.622092. Epub 2011 Sep 8.
Sildenafil provides restorative therapeutic benefits in the treatment of experimental stroke. The majority of experimental studies on treatment of stroke have been performed in young animals; however, stroke is primarily a disease of the aged. Thus, using MRI, we evaluated the effects of sildenafil treatment of embolic stroke in aged animals.
Aged male Wistar rats (18 months) were subjected to embolic stroke and treated daily with saline (n=10) or with sildenafil (n=10) initiated at 24 hours and subsequently for 7 days after onset of ischemia. MRI measurements were performed at 24 hours and weekly to 6 weeks after embolization.
MRI and histological measurements demonstrated that sildenafil treatment of aged rats significantly enhanced angiogenesis and axonal remodeling after stroke compared to saline-treated aged rats. Local cerebral blood flow in the angiogenic area was elevated and expansion of the ipsilateral ventricle and, consequently, brain atrophy was significantly reduced in the sildenafil-treated rats.
Treatment of embolic stroke in aged rats with sildenafil significantly augments angiogenesis and axonal remodeling, which increased local blood flow and reduced expansion of the ipsilateral ventricle 6 weeks after stroke compared to control aged rats. MRI can be used to investigate brain repair after stroke in aged rats.
西地那非在治疗实验性中风方面提供了恢复性治疗益处。大多数关于中风治疗的实验研究都是在年轻动物中进行的；然而，中风主要是老年人的疾病。因此，我们使用 MRI 评估了西地那非治疗栓塞性中风对老年动物的影响。
雄性 Wistar 大鼠（18 个月）被诱发栓塞性中风，并在中风发作后 24 小时开始每天接受生理盐水（n=10）或西地那非（n=10）治疗，随后持续 7 天。MRI 测量在栓塞后 24 小时和每周进行至 6 周。
MRI 和组织学测量表明，与生理盐水治疗的老年大鼠相比，西地那非治疗老年大鼠在中风后显著增强了血管生成和轴突重塑。在血管生成区域的局部脑血流增加，并且在西地那非治疗的大鼠中，对侧脑室的扩张和因此引起的脑萎缩显著减少。
西地那非治疗老年大鼠的栓塞性中风显著增强了血管生成和轴突重塑，与对照老年大鼠相比，6 周后增加了局部脑血流并减少了对侧脑室的扩张。MRI 可用于研究老年大鼠中风后的脑修复。
