Magray Mohammed Siraj Ud Din, Kumar Anup, Rawat Anil Kumar, Srivastava Shipra
Bioinformation. 2011;6(10):370-1. doi: 10.6026/97320630006370. Epub 2011 Aug 2.
A bacterial strain, designated BzDS03 was isolated from water sample, collected from Dal Lake Srinagar. The strain was characterized by using 16S ribosomal RNA gene and 16S-23S rRNA internal transcribed spacer region sequences. Phylogenetic analysis showed that 16S rRNA sequence of the isolate formed a monophyletic clade with genera Escherichia. The closest phylogenetic relative was Escherichia coli with 99% 16S rRNA gene sequence similarity. The result of Ribosomal database project's classifier tool revealed that the strain BzDS03 belongs to genera Escherichia.16S rRNA sequence of isolate was deposited in GenBank with accession number FJ961336. Further analysis of 16S-23S rRNA sequence of isolate confirms that the identified strain BzDS03 be assigned as the type strain of Escherichia coli with 98% 16S-23S rRNA sequence similarity. The GenBank accession number allotted for 16S-23S rRNA intergenic spacer sequence of isolate is FJ961337.
从斯利那加达尔湖采集的水样中分离出一株名为BzDS03的细菌菌株。通过使用16S核糖体RNA基因和16S - 23S rRNA内部转录间隔区序列对该菌株进行了表征。系统发育分析表明，该分离株的16S rRNA序列与埃希氏菌属形成一个单系分支。最接近的系统发育亲缘关系是与大肠杆菌，16S rRNA基因序列相似度为99%。核糖体数据库项目的分类工具结果显示，菌株BzDS03属于埃希氏菌属。分离株的16S rRNA序列已存入GenBank，登录号为FJ961336。对分离株16S - 23S rRNA序列的进一步分析证实，鉴定出的菌株BzDS03被指定为大肠杆菌的模式菌株，16S - 23S rRNA序列相似度为98%。分配给分离株16S - 23S rRNA基因间隔序列的GenBank登录号为FJ961337。
