抗坏血酸和对乙酰氨基酚作为抗生物膜和抗毒力剂对抗耐药菌的显著效果

The Promising Effect of Ascorbic Acid and Paracetamol as Anti-Biofilm and Anti-Virulence Agents against Resistant .

作者信息

Eltabey Sara M, Ibrahim Ali H, Zaky Mahmoud M, Ibrahim Adel Ehab, Alrashdi Yahya Bin Abdullah, El Deeb Sami, Saleh Moustafa M

机构信息

Microbiology Program, Botany Department, Faculty of Science, Port Said University, Port Said 42521, Egypt.

Botany Department, Faculty of Science, Port Said University, Port Said 42521, Egypt.

出版信息

Curr Issues Mol Biol. 2024 Jul 2;46(7):6805-6819. doi: 10.3390/cimb46070406.

is a major cause of serious infections, with antibiotic resistance rendering many treatments ineffective. Hence, novel strategies to combat this pathogen are needed. Anti-virulence therapy is a promising new approach for the subsequent era. Recent research has examined the impact of sub-inhibitory doses of ascorbic acid and paracetamol on virulence factors. This study evaluated biofilm formation, protease production, motility behavior, serum resistance, expression of virulence-regulating genes (using RT-PCR), and survival rates in a mouse model. Ascorbic acid significantly reduced biofilm formation, protease production, motility, and serum resistance from 100% in untreated isolates to 22-89%, 10-89%, 2-57%, and 31-35% in treated isolates, respectively. Paracetamol also reduced these factors from 100% in untreated isolates to 16-76%, 1-43%, 16-38%, and 31-35%, respectively. Both drugs significantly down-regulated virulence-regulating genes , , , , , and . Mice treated with these drugs had a 100% survival rate compared with 60% in the positive control group control inoculated with untreated bacteria. This study highlights the potential of ascorbic acid and paracetamol as anti-virulence agents, suggesting their use as adjunct therapies alongside conventional antimicrobials or as alternative treatments for resistant infections.

是严重感染的主要原因，抗生素耐药性使许多治疗方法无效。因此，需要新的策略来对抗这种病原体。抗毒力疗法是后续时代一种有前景的新方法。最近的研究探讨了亚抑制剂量的抗坏血酸和对乙酰氨基酚对毒力因子的影响。本研究评估了生物膜形成、蛋白酶产生、运动行为、血清抗性、毒力调节基因的表达（使用逆转录聚合酶链反应）以及在小鼠模型中的存活率。抗坏血酸显著降低了生物膜形成、蛋白酶产生、运动性和血清抗性，在未处理的分离株中这些指标为100%，而在处理过的分离株中分别降至22 - 89%、10 - 89%、2 - 57%和31 - 35%。对乙酰氨基酚也将这些指标从未处理分离株中的100%分别降至16 - 76%、1 - 43%、16 - 38%和31 - 35%。两种药物均显著下调了毒力调节基因、、、、和。用这些药物治疗的小鼠存活率为100%，而接种未处理细菌的阳性对照组存活率为60%。本研究突出了抗坏血酸和对乙酰氨基酚作为抗毒力药物的潜力，表明它们可作为传统抗菌药物的辅助疗法或作为耐药感染的替代治疗方法。