Devaraj Venkatapura C, Krishna Burdipad G, Viswanatha Gollapalle L
Department of Pharmacology, Bioneeds Laboratory Animals and Preclinical Services, Bangalore, India.
Zhong Xi Yi Jie He Xue Bao. 2011 Sep;9(9):1022-30. doi: 10.3736/jcim20110914.
To develop a rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to analyze quercetin (QU), rutin (RU) and kaempferol (KA) simultaneously in the leaf extracts of Moringa oleifera Lam. and Raphinus sativus Linn.
Samples were prepared by extracting the leaves of the M. oleifera and R. sativus by cold-maceration technique using 90% ethanol. Chromatographic separation was operated with a mixture of 0.2% formic acid in water and acetonitrile at a flow rate of 0.4 mL/min on a Phenomenex Gemini C18 column with a total run time of 5.01 min.
The MS/MS ion transitions monitored were 303.03 to 153.1 for QU, 611.1 to 303.1 for RU, 287.1 to 153.2 for KA and 180.1 to 110.1 for internal standard. The lower limit of quantitation achieved for QU, RU and KA was 5 ng/mL and the linearity was observed from 5 to 2 000 ng/mL. The correlation coefficients of linear regression analysis were 0.994 6, 0.995 1 and 0.996 9 for QU, RU and KA, respectively.
The results indicate that the LC-MS/MS method is fast and sensitive and may provide excellent specificity for simultaneous determination of QU, RU and KA in leaf extracts of M.oleifera and R.sativus.
建立一种快速灵敏的液相色谱-串联质谱（LC-MS/MS）方法，用于同时分析辣木（Moringa oleifera Lam.）和萝卜（Raphinus sativus Linn.）叶片提取物中的槲皮素（QU）、芦丁（RU）和山奈酚（KA）。
采用冷浸法，用90%乙醇提取辣木和萝卜的叶片来制备样品。在Phenomenex Gemini C18柱上，以0.2%甲酸水溶液和乙腈的混合溶液为流动相，流速为0.4 mL/min进行色谱分离，总运行时间为5.01分钟。
监测到的MS/MS离子跃迁为：QU的303.03到153.1、RU的611.1到303.1、KA的287.1到153.2以及内标的180.1到110.1。QU、RU和KA的定量下限为5 ng/mL，线性范围为5到2000 ng/mL。QU、RU和KA线性回归分析的相关系数分别为0.994 6、0.995 1和0.996 9。
结果表明，LC-MS/MS方法快速灵敏，可用于同时测定辣木和萝卜叶片提取物中的QU、RU和KA，具有良好的特异性。
