Chinese Academy of Tropical Agricultural Sciences Center for Food Quality Supervision and Testing Ministry of Agriculture (Zhanjiang), No. 48 Renmindadaonan, Zhanjiang 524001, China; Agricultural Product Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, No. 48 Renmindadaonan, Zhanjiang 524001, China.
Agricultural Product Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, No. 48 Renmindadaonan, Zhanjiang 524001, China.
Food Chem. 2016 Sep 15;207:157-61. doi: 10.1016/j.foodchem.2016.03.089. Epub 2016 Mar 30.
A new method for simultaneous determination of eight vitamin E isomers including α-, β-, γ-, and δ-tocopherol and α-, β-, γ-, and δ-tocotrienol by ultra-performance convergence chromatography (UPC(2)) equipped with a diode array detector was reported. They were separated on a BEH 2-EP column (3.0 mm × 100 mm, 1.7 μm) using gradient elution (95:5-80:20) with a mobile phase consisted of CO2 and methanol:isopropanol (1:1, v/v), back pressure of 1800 psi, flow rate of 1.5 ml/min and detection at 294 nm. The results showed good linearity (R(2)=0.9990-0.9998) and high resolution (1.48-7.67). Limits of detection (LOD) and quantification (LOQ) ranged from 23-49 ng/L and 70-150 ng/L, respectively. Relative standard deviations (RSD) for repeatability and reproducibility were 0.62-3.16% and 0.82-3.34%, respectively. Moreover, this method was successfully applied to analysis the vitamin E isomers in Moringa oleifera leaf samples.
一种利用超高效合相色谱（UPC(2)）结合二极管阵列检测器同时测定 8 种维生素 E 异构体（包括 α-、β-、γ-、δ-生育酚和 α-、β-、γ-、δ-生育三烯酚）的新方法。在 BEH 2-EP 柱（3.0mm×100mm，1.7μm）上，采用 CO2 和甲醇：异丙醇（1:1，v/v）作为流动相进行梯度洗脱（95:5-80:20），背压为 1800psi，流速为 1.5ml/min，检测波长为 294nm。结果表明，该方法具有良好的线性（R(2)=0.9990-0.9998）和高分辨率（1.48-7.67）。检测限（LOD）和定量限（LOQ）分别为 23-49ng/L 和 70-150ng/L。重复性和再现性的相对标准偏差（RSD）分别为 0.62-3.16%和 0.82-3.34%。此外，该方法成功应用于分析辣木叶样品中的维生素 E 异构体。
