Panchal Hiteksha, Shah Mamta B
Anand Pharmacy College, Department of Pharmacognosy, Anand, Gujarat, India.
L. M. College of Pharmacy, Department of Pharmacognosy, Ahmedabad, Gujarat, India.
J AOAC Int. 2017 Jul 1;100(4):971-975. doi: 10.5740/jaoacint.16-0416. Epub 2017 Mar 16.
In this study, a simple and rapid LC with tandem MS method was developed and validated for the simultaneous determination of kaempferol and quercetin in Thespesia populnea extract. The compounds were eluted using a Gemini C18 column (50 × 2.0 mm, 3 μm), with the mobile phase consisting of acetonitrile-0.3% formic acid in water at the flow rate of 0.400 mL/min. The assay exhibited a linear dynamic range of 25-2500 ng/mL for both kaempferol and quercetin. The values for intra- and interday precision and accuracy were well within the generally accepted criteria for analytical methods (<15%). Selectivity, linearity, LOD, LOQ, accuracy, and precision were evaluated for both analytes. The proposed method is accurate and sensitive and can be used for the routine quantification of kaempferol and quercetin in the herbal extract and in polyherbal formulations.
在本研究中，开发并验证了一种简单快速的液相色谱串联质谱法，用于同时测定杨叶肖槿提取物中的山柰酚和槲皮素。使用Gemini C18柱（50×2.0 mm，3μm）洗脱化合物，流动相由乙腈-0.3%甲酸水溶液组成，流速为0.400 mL/min。该测定法对山柰酚和槲皮素的线性动态范围均为25-2500 ng/mL。日内和日间精密度及准确度值均在分析方法普遍接受的标准范围内（<15%）。对两种分析物的选择性、线性、检测限、定量限、准确度和精密度进行了评估。所提出的方法准确、灵敏，可用于草药提取物和多草药配方中山柰酚和槲皮素的常规定量分析。
