Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan, ROC.
Anaerobe. 2011 Oct;17(5):239-45. doi: 10.1016/j.anaerobe.2011.08.001. Epub 2011 Aug 28.
To investigate the frequency of tetracycline resistance (Tet-R) lactobacilli in pig intestines, a total of 256 pig colons were analyzed and found to contain typical colonies of Tet-R lactic acid bacteria in every sample, ranging from 3.2 × 10(3) to 6.6 × 10(5) CFU/cm(2). From these samples, a total of 159 isolates of Tet-R lactobacilli were obtained and identified as belonging to 11 species, including Lactobacillus reuteri, Lactobacillus amylovorus, Lactobacillus salivarius, Lactobacillus plantarum, Lactobacillus ruminis, Lactobacillus kefiri, Lactobacillus fermentum, Lactobacillus sakei, Lactobacillus coryniformis, Lactobacillus parabuchneri and Lactobacillus letivazi. Based on the EFSA (2008) breakpoints, all isolates, after MIC analysis, were qualified as Tet-R, from which the significant high Tet-R MIC(50) and MIC(90) values indicated an ecological distribution of Tet-R lactobacilli mostly with high resistance potency in pig colons. PCR-detection identified 5 tet genes in these isolates, the most predominant one being tet (W), followed by tet (M), (L), (K), and (Q). Their detection rates were 82.0%, 22.5%, 14.4%, 8.1% and 0.9%, respectively. Noteworthily, isolates of the same species carrying identical tet gene(s) usually had a wide different MIC values. Furthermore, strain-subtyping of these isolates by REP-PCR demonstrated a notable genotypic biodiversity % (average = 62%).
为了研究猪肠道中四环素耐药（Tet-R）乳酸菌的频率，分析了总共 256 个猪结肠，发现每个样本中都含有典型的 Tet-R 乳酸菌菌落，范围从 3.2×10(3)到 6.6×10(5)CFU/cm(2)。从这些样本中，共获得了 159 株 Tet-R 乳酸菌分离株，并鉴定为 11 个种，包括乳酸乳球菌、淀粉乳杆菌、唾液乳杆菌、植物乳杆菌、瘤胃乳杆菌、开菲尔乳杆菌、发酵乳杆菌、清酒乳杆菌、乳杆菌、副干酪乳杆菌和嗜酸乳杆菌。根据 EFSA（2008 年）的断点，所有分离株在 MIC 分析后均被确认为 Tet-R，其中 Tet-R MIC(50)和 MIC(90)值较高，表明 Tet-R 乳酸菌在猪结肠中的生态分布主要具有较高的耐药能力。PCR 检测在这些分离株中鉴定出 5 个 tet 基因，其中最主要的是 tet (W)，其次是 tet (M)、(L)、(K)和 (Q)。它们的检出率分别为 82.0%、22.5%、14.4%、8.1%和 0.9%。值得注意的是，同一物种的分离株携带相同的 tet 基因通常具有广泛不同的 MIC 值。此外，这些分离株的菌株分型通过 REP-PCR 表明具有显著的基因型生物多样性（平均值=62%）。
