鸡源分离株的抗生素敏感性评估。
Assessment of antibiotic susceptibility in isolates from chickens.
作者信息
Dec Marta, Urban-Chmiel Renata, Stępień-Pyśniak Dagmara, Wernicki Andrzej
机构信息
Sub-Department of Veterinary Prevention and Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland.
出版信息
Gut Pathog. 2017 Sep 19;9:54. doi: 10.1186/s13099-017-0203-z. eCollection 2017.
BACKGROUND
The aim of this study was to determine the susceptibility of 88 isolates derived from chickens to antibiotic substances and to detect drug-resistance genes.
RESULTS
The minimal inhibitory concentration of 13 antimicrobial substances was determined by the broth microdilution method, and resistance genes were detected by PCR. We recorded a high prevalence of resistance to tiamulin (90% resistant isolates), tetracyclines (74%) and lincomycin (70%), and a moderately high frequency of resistance to enrofloxacin (48%), macrolides (42%), aminoglycosides (12.5-31%), ampicillin (26%) and chloramphenicol (23%). Multi-drug resistance was observed in 79.5% of isolates. The presence of resistance genes was generally correlated with phenotypic resistance, but some molecular determinants were also recorded in susceptible isolates. Among tetracycline resistance genes, the most frequently identified was (45% isolates), followed by (26%) and (24%). The , and genes, associated with resistance to macrolides and lincosamides, were observed in 39, 12 and 39% of isolates, respectively. Among genes determining resistance to aminoglycoside antibiotics, we identified - (10% of isolates), '--'- (8%), ″- (6%) and (4.5%). The gene was present in 32 isolates, including 8 of 20 found to be resistant to chloramphenicol. Two genes encoding efflux pumps were identified-the gene was present in all isolates tested, and 10 of 79 lactobacilli determined to be phenotypically resistant to tiamulin contained the gene. We were unable to explain the resistance mechanism of isolates to ampicillin, but showed that it did not involve the production of β-lactamases.
CONCLUSIONS
Our findings indicate that intestinal lactobacilli should be considered a reservoir of resistance genes and that antibiotics must be used prudently in poultry production. The data derived from this study can be used as a basis for reviewing current microbiological breakpoints for categorization of susceptible and resistant strains within the genus .
背景
本研究旨在确定从鸡中分离出的88株菌株对抗生素物质的敏感性，并检测耐药基因。
结果
采用肉汤微量稀释法测定了13种抗菌物质的最低抑菌浓度，并通过聚合酶链反应（PCR）检测耐药基因。我们记录到对泰妙菌素（90%的分离株耐药）、四环素（74%）和林可霉素（70%）的耐药率很高，对恩诺沙星（48%）、大环内酯类（42%）、氨基糖苷类（12.5 - 31%）、氨苄西林（26%）和氯霉素（23%）的耐药频率中等偏高。79.5%的分离株观察到多重耐药。耐药基因的存在通常与表型耐药相关，但在敏感分离株中也记录到一些分子决定因素。在四环素耐药基因中，最常鉴定出的是（45%的分离株），其次是（26%）和（24%）。与大环内酯类和林可酰胺类耐药相关的、和基因，分别在39%、12%和39%的分离株中观察到。在决定对氨基糖苷类抗生素耐药的基因中，我们鉴定出 - （10%的分离株）、'--'- （8%）、″- （6%）和（4.5%）。基因存在于32株分离株中，包括20株对氯霉素耐药的分离株中的8株。鉴定出两个编码外排泵的基因 - 基因存在于所有测试的分离株中，79株对泰妙菌素表型耐药的乳酸杆菌中有10株含有基因。我们无法解释分离株对氨苄西林的耐药机制，但表明其不涉及β - 内酰胺酶的产生。
结论
我们的研究结果表明，肠道乳酸杆菌应被视为耐药基因的储存库，并且在家禽生产中必须谨慎使用抗生素。本研究获得的数据可作为审查当前微生物学断点以对属内敏感和耐药菌株进行分类的基础。
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