Rhodes Shelley G, Gunn-Mooore Danielle, Boschiroli Maria Laura, Schiller Irene, Esfandiari Javan, Greenwald Rena, Lyashchenko Konstantin P
TB Research Group, Animal Health and Veterinary Laboratories Agency, Surrey KT15 3NB, United Kingdom.
Vet Immunol Immunopathol. 2011 Nov 15;144(1-2):129-34. doi: 10.1016/j.vetimm.2011.07.020. Epub 2011 Jul 30.
This study describes the comparison of the cell-based interferon-gamma (IFNγ) test with serological rapid antibody tests (STAT-PAK and DPP VetTB) for the ante mortem testing of tuberculosis in domestic cats. The antibody specificities of rapid antibody test-positive cats were further discerned using multi-antigen print immunoassay. A total of 62 cats with culture-confirmed Mycobacterium bovis, Mycobacterium microti, Mycobacterium avium and Mycobacterium malmoense, as well as negative controls and dangerous-contact cats were tested. Tests were also applied longitudinally to one further cat undergoing TB chemotherapy for suspected M. bovis infection. Our data from this small study show excellent test specificity (100% for all tests) and encouraging levels of test sensitivity for M. bovis and TB Complex infections (IFNγ 70-100% depending upon test interpretation criteria; rapid tests both 90% for M. bovis infection and up to 46.2% for M. microti infection). The differential diagnosis of very pathogenic TB Complex (M. bovis, Mycobacterium tuberculosis), as opposed to less-pathogenic TB Complex (M. microti) was possible where positive responses to the protein cocktail ESAT6/CFP10 were observed (80% of M. bovis-infected cats in this study showed positive IFNγ responses to ESAT6/CFP10, while 20% had antibody responses to ESAT6/CFP10 using MAPIA). Finally, preliminary data from a longitudinal study of one M. bovis-exposed cat with a positive IFNγ test pre-treatment suggest that a decrease in bacterial burden may be reflected in the IFNγ response, and thus the IFNγ test may provide a monitor for TB chemotherapy.
本研究描述了基于细胞的干扰素-γ（IFNγ）检测与血清学快速抗体检测（STAT-PAK和DPP VetTB）在家猫生前结核病检测中的比较。使用多抗原印迹免疫分析法进一步鉴别快速抗体检测呈阳性的猫的抗体特异性。对总共62只经培养确诊感染牛分枝杆菌、微小分枝杆菌、鸟分枝杆菌和马尔默分枝杆菌的猫，以及阴性对照猫和密切接触危险的猫进行了检测。还对另一只疑似感染牛分枝杆菌且正在接受结核病化疗的猫进行了纵向检测。我们这项小型研究的数据显示，检测具有出色的特异性（所有检测的特异性均为100%），对于牛分枝杆菌和结核复合群感染，检测灵敏度水平令人鼓舞（IFNγ检测的灵敏度为70%-100%，具体取决于检测解读标准；快速检测对于牛分枝杆菌感染的灵敏度均为90%，对于微小分枝杆菌感染的灵敏度高达46.2%）。在观察到对蛋白混合物ESAT6/CFP10呈阳性反应的情况下，有可能对致病性很强的结核复合群（牛分枝杆菌、结核分枝杆菌）与致病性较弱的结核复合群（微小分枝杆菌）进行鉴别诊断（在本研究中，80%感染牛分枝杆菌的猫对ESAT6/CFP10的IFNγ反应呈阳性，而20%使用多抗原点免疫分析对ESAT6/CFP10有抗体反应）。最后，对一只暴露于牛分枝杆菌且IFNγ检测预处理呈阳性的猫进行纵向研究的初步数据表明，细菌负荷的降低可能反映在IFNγ反应中，因此IFNγ检测可能为结核病化疗提供监测指标。
