IT Convergence Technology Research Lab., ETRI, Daejeon 305-700, Republic of Korea.
J Neurosci Methods. 2011 Oct 30;202(1):38-44. doi: 10.1016/j.jneumeth.2011.08.036. Epub 2011 Aug 31.
We have prepared the poly-d-lysine (PDL) bound surfaces for neuron cell culture by covalent binding between the poly-d-Lysine and substrates and investigated neuronal cell adhesion properties and cell growth morphology. The number of neuronal cell and the number of neurite per neuronal cell on PDL bound surfaces was much more than those on PDL coated surfaces and also the neuronal cells on PDL bounded surfaces survived a longer time. On the pattern of covalently bound PDL, neuronal cells and their neurites are confined within the grid line leading to patterned neuronal networks with the long-term survival.
我们通过聚赖氨酸（PDL）与基底的共价结合，制备了用于神经元细胞培养的 PD L 结合表面，并研究了神经元细胞的黏附特性和细胞生长形态。与 PD L 涂覆表面相比，PDL 结合表面上神经元细胞的数量和每个神经元细胞的神经突数量都多得多，而且 PDL 结合表面上的神经元细胞存活时间也更长。在共价结合的 PDL 图案上，神经元细胞及其神经突被限制在网格线内，形成具有长期存活能力的图案化神经元网络。
