Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea.
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea.
J Neurosci Methods. 2012 Feb 15;204(1):28-34. doi: 10.1016/j.jneumeth.2011.10.023. Epub 2011 Oct 29.
A variety of materials has been applied to neuronal cell culture substrates to improve the efficiency of the culture and to provide pertinent cell growth environment. Here we report the application of Parafilm(®) M ('Parafilm') as a novel substrate for neuronal culture and patterning. Cell culture results show that elastic Parafilm had effects on cell viability, length and number of neurites, and soma spreading. Parafilm was also an effective substrate to obtain patterned neuronal cultures using a conventional micro-contract printing (μCP) technique. Polylysine micropatterns in line or grid forms were readily transferred from PDMS stamp to bare Parafilm surfaces and spatially confined neuronal cultures were successfully maintained for over three weeks. We also demonstrate that batch-processing cell culture substrates can be easily fabricated using a piece of Parafilm. The softness, plasticity, and hydrophobicity were main features that made it attractive for Parafilm to be considered as a practical cell culture platform. The results can be extended to develop an inexpensive and practical neuronal culture substrates in tissue engineering and biochip applications.
多种材料已被应用于神经元细胞培养的基底上，以提高培养效率并提供相关的细胞生长环境。在这里，我们报告了 Parafilm(®) M（'Parafilm'）作为神经元培养和图案化的新型基底的应用。细胞培养结果表明，弹性 Parafilm 对细胞活力、突起的长度和数量以及细胞体的伸展有影响。Parafilm 也是一种有效的基底，可以使用传统的微接触印刷（μCP）技术获得图案化的神经元培养物。聚赖氨酸的线条或网格形式的微图案很容易从 PDMS 印章转移到裸露的 Parafilm 表面，并且成功地维持了空间受限的神经元培养物超过三周。我们还证明，使用一块 Parafilm 可以轻松地制造批量处理的细胞培养基底。柔软性、可塑性和疏水性是使其成为实用细胞培养平台的有吸引力的主要特征。这些结果可扩展到组织工程和生物芯片应用中开发廉价且实用的神经元培养基底。
