Institute of Biotechnology, College of Animal Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
Res Vet Sci. 2012 Aug;93(1):544-8. doi: 10.1016/j.rvsc.2011.08.004. Epub 2011 Sep 9.
Bovine viral diarrhea virus (BVDV) is one of the most important pathogens to the cattle industry, causing a significant economic loss throughout the world. Despite the wide use of various control measures for BVDV, the disease remains prevalent. In this study, we achieved an efficient inhibition of NADL strain replication by plasmid-mediated shRNA targeting conserved regions of the viral genome. To further enhance the inhibiting efficiency, a dual shRNA expression plasmid, which could simultaneously express two different shRNA, was established and showed stronger inhibitory effects on virus replication. Moreover, the antiviral activity induced by the dual shRNA expression system was also evident on other BVDV-1 subgenotypes (BVDV-1a, BVDV-1b and BVDV-1c). Therefore, the dual shRNA system provides a more powerful strategy for inhibiting BVDV replication in a cross-resistance manner.
牛病毒性腹泻病毒（BVDV）是畜牧业最重要的病原体之一，在全球范围内造成了巨大的经济损失。尽管广泛应用了各种 BVDV 控制措施，但该疾病仍然普遍存在。在这项研究中，我们通过针对病毒基因组保守区域的质粒介导 shRNA 实现了对 NADL 株复制的有效抑制。为了进一步提高抑制效率，构建了能够同时表达两种不同 shRNA 的双 shRNA 表达质粒，该质粒对病毒复制表现出更强的抑制作用。此外，双 shRNA 表达系统诱导的抗病毒活性也在其他 BVDV-1 亚群（BVDV-1a、BVDV-1b 和 BVDV-1c）中得到证实。因此，双 shRNA 系统为以交叉抗性方式抑制 BVDV 复制提供了更强大的策略。
