Department of Engineering Sciences, Division of Nanotechnology and Functional Materials, Uppsala University, The Ångström Laboratory, Box 534, SE-751 21 Uppsala, Sweden.
Biosens Bioelectron. 2011 Nov 15;29(1):195-9. doi: 10.1016/j.bios.2011.08.019. Epub 2011 Aug 22.
Here, the volume-amplified magnetic nanobead detection assay (VAM-NDA) is for the first time applied for detection of rolling circle amplified (RCA) DNA molecules in a portable, commercial AC susceptometer that operates at ambient temperatures and with an analysis time of about 20 min. The performance of the assay is investigated using three different magnetic nanobead sizes: 50, 130 and 250nm. The performance of the assay using the AC susceptometer is compared to the performance achieved using a superconducting quantum interference device (SQUID). It is found that the performance of the assay is comparable in the two setups with a quantitative detection limit of ∼4pM for all bead sizes under study. The findings show that the VAM-NDA holds promise for future wide-spread implementation in commercial AC susceptometer setups thus opening up for the possibility to perform magnetic bead-based DNA detection in point-of-care and outpatient settings.
这里，体积放大的磁性纳米珠检测法（VAM-NDA）首次应用于在便携式、商用交流磁化率计中检测滚环扩增（RCA）DNA 分子，该磁化率计在环境温度下运行，分析时间约为 20 分钟。使用三种不同的磁性纳米珠尺寸：50nm、130nm 和 250nm 来研究该检测法的性能。使用交流磁化率计进行的检测法的性能与使用超导量子干涉装置（SQUID）获得的性能进行了比较。结果发现，在两种设置中，该检测法的性能相当，对于所有研究的纳米珠尺寸，定量检测限约为 4pM。研究结果表明，VAM-NDA 有望在未来在商用交流磁化率计设置中得到广泛应用，从而为在即时护理和门诊环境中进行基于磁珠的 DNA 检测开辟了可能性。
