Strömberg M, Zardán Gómez de la Torre T, Göransson J, Gunnarsson K, Nilsson M, Strømme M, Svedlindh P
Department of Engineering Sciences, Division of Nanotechnology and Functional Materials, Uppsala University, The Angström Laboratory, Box 534, SE-751 21 Uppsala, Sweden.
Biosens Bioelectron. 2008 Dec 1;24(4):696-703. doi: 10.1016/j.bios.2008.06.043. Epub 2008 Jul 6.
The volume amplified magnetic nanobead detection assay [Strömberg, M., Göransson, J., Gunnarsson, K., Nilsson, M., Svedlindh, P., Strømme, M., 2008. Nano Letters 8, 816-821] was investigated with respect to bead size, bead surface coverage of probe oligonucleotides, bead concentration and rolling circle amplification (RCA) time, with the objective to improve the understanding of the microscopic mechanisms influencing the assay. The most important findings for future biosensor development were the following: (i) small beads exhibit a much reduced tendency to cross-link several RCA products, thus enabling use of both complex magnetisation turn-on and turn-off detection strategies, whereas larger beads only allow for turn-off detection; (ii) small beads exhibit faster immobilisation kinetics, thus reducing the time for diagnostic test completion, and also immobilise in larger numbers than larger beads. Finally, (iii) by demonstrating qualitative dual-target detection of bacterial DNA sequences using 130 and 250nm beads, the bioassay was shown to allow for multiplexed detection.
针对体积放大磁纳米珠检测分析方法[斯特伦贝里，M.，戈兰松，J.，贡纳松，K.，尼尔松，M.，斯韦德林德，P.，斯特勒姆，M.，2008年。《纳米快报》8，816 - 821]，研究了磁珠大小、探针寡核苷酸的磁珠表面覆盖率、磁珠浓度和滚环扩增（RCA）时间，目的是增进对影响该分析方法的微观机制的理解。未来生物传感器开发的最重要发现如下：（i）小磁珠交联多个RCA产物的倾向大大降低，因此既可以使用复杂的磁化开启和关闭检测策略，而大磁珠仅允许关闭检测；（ii）小磁珠表现出更快的固定动力学，从而减少了诊断测试完成时间，并且比大磁珠固定的数量更多。最后，（iii）通过使用130和250nm磁珠对细菌DNA序列进行定性双靶标检测，表明该生物分析方法可进行多重检测。
