Hashem Mahmoud A, Alotaibi Badriyah S, Elsayed Mahmoud M A, Alosaimi Manal E, Hussein Amal K, Abduljabbar Maram H, Lee Kyung-Tae, Abdelkader Hamdy, El-Mokhtar Mohamed A, Hassan Ahmed H E, Abdel-Rheem Amany A, Belal Amany, Saddik Mohammed S
Department of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Sohag University, Sohag 82524, Egypt.
Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
Pharmaceutics. 2023 Oct 4;15(10):2423. doi: 10.3390/pharmaceutics15102423.
Wound healing is a significant healthcare problem that decreases the patient's quality of life. Hence, several agents and approaches have been widely used to help accelerate wound healing. The challenge is to search for a topical delivery system that could supply long-acting effects, accurate doses, and rapid healing activity. Topical forms of simvastatin (SMV) are beneficial in wound care. This study aimed to develop a novel topical chitosan-based platform of SMV with folic acid (FA) for wound healing. Moreover, the synergistic effect of combinations was determined in an excisional wound model in rats. The prepared SMV-FA-loaded films (SMV-FAPFs) were examined for their physicochemical characterizations and morphology. Box-Behnken Design and response surface methodology were used to evaluate the tensile strength and release characteristics of the prepared SMV-FAPFs. Additionally, Fourier transform infrared (FT-IR), differential scanning calorimetry (DSC), X-ray diffraction pattern (XRD), and animal studies were also investigated. The developed SMV-FAPFs showed a contraction of up to 80% decrease in the wound size after ten days. The results of the quantitative real-time polymerase chain reaction (RT-PCR) analysis demonstrated a significant upregulation of dermal collagen type I (CoTI) expression and downregulation of the inflammatory JAK3 expression in wounds treated with SMV-FAPFs when compared to control samples and individual drug treatments. In summary, it can be concluded that the utilization of SMV-FAPFs holds great potential for facilitating efficient and expeditious wound healing, hence presenting a feasible substitute for conventional topical administration methods.
伤口愈合是一个严重的医疗保健问题，会降低患者的生活质量。因此，多种药物和方法已被广泛用于帮助加速伤口愈合。挑战在于寻找一种能够提供长效作用、精确剂量和快速愈合活性的局部给药系统。辛伐他汀（SMV）的局部剂型在伤口护理中有益。本研究旨在开发一种新型的基于壳聚糖的含叶酸（FA）的SMV局部给药平台用于伤口愈合。此外，在大鼠切除伤口模型中确定了组合的协同作用。对制备的载SMV-FA的薄膜（SMV-FAPFs）进行了物理化学表征和形态学检查。采用Box-Behnken设计和响应面方法来评估制备的SMV-FAPFs的拉伸强度和释放特性。此外，还进行了傅里叶变换红外（FT-IR）、差示扫描量热法（DSC）、X射线衍射图谱（XRD）和动物研究。所开发的SMV-FAPFs在十天后显示伤口大小收缩高达80%。定量实时聚合酶链反应（RT-PCR）分析结果表明，与对照样品和单独药物治疗相比，用SMV-FAPFs治疗的伤口中真皮I型胶原蛋白（CoTI）表达显著上调，炎症性JAK3表达下调。总之，可以得出结论，使用SMV-FAPFs在促进高效快速伤口愈合方面具有巨大潜力，因此为传统局部给药方法提供了一种可行的替代方案。
