School of Geography, Earth and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA, UK.
Environ Pollut. 2011 Dec;159(12):3504-8. doi: 10.1016/j.envpol.2011.08.018. Epub 2011 Sep 9.
The cytotoxic drug, cisplatin (cis-PtCl(2)(NH(3))(2)), has been added to cultures of the marine macroalga, Ulva lactuca, under various experimental conditions. Both accumulation and internalisation over a 48 h period was greater when cisplatin was added to coastal sea water (salinity = 33) from a distilled water solution than when added to either sea water or estuarine water (salinity = 16.5) from a saline solution. This effect is attributed to the greater abundance of the more reactive monoaqua complex (cis-PtCl(OH(2))(NH(3))(2)(+)) in the distilled water solution and kinetic constraints on its conversion back to cis-PtCl(2)(NH(3))(2) in sea water. Despite its mode of action at the cellular level, cisplatin added up to concentrations of 150 nM did not incur a measurable reduction in the efficiency of photochemical energy conversion under any of experimental conditions tested.
细胞毒性药物顺铂（cis-PtCl(2)(NH(3))(2)）已在各种实验条件下添加到海洋大型藻类浒苔（Ulva lactuca）的培养物中。在 48 小时的时间内，当顺铂从蒸馏水溶液添加到沿海海水（盐度=33）中时，其积累和内化作用大于从盐水溶液添加到海水或河口水中（盐度=16.5）。这种效应归因于在蒸馏水中溶液中更具反应性的单水合配合物（cis-PtCl(OH(2))(NH(3))(2)(+））的丰度更高，以及在海水中其转化回 cis-PtCl(2)(NH(3))(2)的动力学限制。尽管顺铂在细胞水平上的作用方式，但在任何测试的实验条件下，添加高达 150 nM 的顺铂并未导致光化学能量转换效率可测量的降低。
