Król Jaroslaw, Bania Jacek, Florek Magdalena, Pliszczak-Król Aleksandra, Staroniewicz Zdzislaw
Department of Pathology, University of Environmental and Life Sciences, Norwida St. 31, 50-375 Wroclaw, Poland.
J Vet Diagn Invest. 2011 May;23(3):532-7. doi: 10.1177/1040638711403434.
A set of polymerase chain reaction (PCR) assays for identification of the most important Pasteurellaceae species encountered in cats and dogs were developed. Primers for Pasteurella multocida were designed to detect a fragment of the kmt, a gene encoding the outer-membrane protein. Primers specific to Pasteurella canis, Pasteurella dagmatis, and Pasteurella stomatis were based on the manganese-dependent superoxide dismutase gene (sodA) and those specific to [Haemophilus] haemoglobinophilus on species-specific sequences of the 16S ribosomal RNA gene. All the primers were tested on respective reference and control strains and applied to the identification of 47 canine and feline field isolates of Pasteurellaceae. The PCR assays were shown to be species specific, providing a valuable supplement to phenotypic identification of species within this group of bacteria.
开发了一组用于鉴定猫和狗中最常见的巴斯德氏菌科物种的聚合酶链反应（PCR）检测方法。多杀巴斯德氏菌的引物设计用于检测kmt基因的一个片段，该基因编码外膜蛋白。犬巴斯德氏菌、牙龈巴斯德氏菌和口腔巴斯德氏菌的特异性引物基于锰依赖性超氧化物歧化酶基因（sodA），而嗜血红蛋白巴斯德氏菌的特异性引物基于16S核糖体RNA基因的物种特异性序列。所有引物均在各自的参考菌株和对照菌株上进行了测试，并应用于47株犬科和猫科巴斯德氏菌科野外分离株的鉴定。这些PCR检测方法具有物种特异性，为该组细菌内物种的表型鉴定提供了有价值的补充。
