Veterinary Medical Research Institute of the Hungarian Academy of Sciences, H-1581 Budapest, Hungary.
Diagn Microbiol Infect Dis. 2011 Sep;71(1):6-11. doi: 10.1016/j.diagmicrobio.2011.04.016. Epub 2011 Jul 26.
The zoonotic impact of Pasteurella species in human wounds caused by cats and dogs has increased recently. In this study, the effectiveness of the Biolog Microstation ID System (Biolog, Hayward, CA) for the identification of certain species of Pasteurella sensu stricto was analysed. Thirty-eight isolates originating from dogs and cats were studied by pheno- and genotypic methods. The classical biochemical tests identified these isolates as P. multocida, P. dagmatis, and P. canis, while the Biolog system distinguished only 2 categories: P. multocida and P. dagmatis. The sodA gene sequence-based phylogenetic analysis revealed that the isolates identified as P. dagmatis by the Biolog system were either P. dagmatis, P. canis, or P. dagmatis-like genomospecies. The low discrimination power of the Biolog system in the case of these closely related Pasteurella species draws attention to the need of continuously improving the database of automated microbial identification systems.
最近，由猫和狗引起的人伤口中巴氏杆菌属物种的人畜共患病影响有所增加。在这项研究中，分析了生物梅里埃 Microstation ID 系统（Biolog，海沃德，加利福尼亚）用于鉴定某些严格意义上的巴氏杆菌属物种的有效性。通过表型和基因型方法研究了 38 株源自狗和猫的分离株。经典生化试验将这些分离株鉴定为多杀巴斯德菌、支气管败血波氏杆菌和犬巴斯德菌，而生物梅里埃系统仅区分出 2 个类别：多杀巴斯德菌和支气管败血波氏杆菌。基于 sodA 基因序列的系统发育分析表明，生物梅里埃系统鉴定为支气管败血波氏杆菌的分离株要么是支气管败血波氏杆菌、犬巴斯德菌，要么是类似于支气管败血波氏杆菌的基因组种。在这些密切相关的巴氏杆菌属物种的情况下，生物梅里埃系统的低鉴别能力引起了对不断改进自动化微生物鉴定系统数据库的需求。
