Obst M, Cornelissen A W
Max-Planck-Institut für Biologie, Molecular Parasitology Unit, Tübingen, Federal Republic of Germany.
Histochemistry. 1990;94(1):101-7. doi: 10.1007/BF00266796.
A non-radioactive in situ hybridization technique, used to map unique DNA sequences to plant chromosomes, has been adapted for the localization of specific DNA sequences in nuclei of Plasmodium berghei. After hybridization using probes labeled with biotin-11-dUTP, the formed DNA/DNA hybrids were detected by fluorescence microscopy using a specific double-layer antibody technique. Besides its high resolution, this procedure is characterized by a high sensitivity, allowing the detection of a unique sequence as small as 2.5 kb.
一种用于将独特DNA序列定位到植物染色体上的非放射性原位杂交技术，已被改编用于定位伯氏疟原虫细胞核中的特定DNA序列。使用生物素-11-dUTP标记的探针进行杂交后，通过特定的双层抗体技术利用荧光显微镜检测形成的DNA/DNA杂交体。除了具有高分辨率外，该方法还具有高灵敏度的特点，能够检测到小至2.5 kb的独特序列。
